Activating Compound | Comment | Organism | Structure |
---|---|---|---|
ascorbate | activates, best at 0.7 mM | Lepidium latifolium | |
DTT | activates at 0.5-5 mM | Lepidium latifolium | |
EDTA | - |
Lepidium latifolium | |
glutathione | GSH, reduced forms of the enzyme are more active | Lepidium latifolium | |
Urea | - |
Lepidium latifolium |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ba2+ | - |
Lepidium latifolium | |
Ca2+ | - |
Lepidium latifolium | |
Cu2+ | 25% inhibition at 10 mM | Lepidium latifolium | |
D-glucose | - |
Lepidium latifolium | |
Mg2+ | - |
Lepidium latifolium | |
Mn2+ | - |
Lepidium latifolium | |
SDS | - |
Lepidium latifolium | |
Zn2+ | - |
Lepidium latifolium |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.162 | - |
sinigrin | pH 6.0, 37°C, with 1 mM GSH | Lepidium latifolium | |
0.171 | - |
sinigrin | pH 6.0, 37°C, without GSH | Lepidium latifolium |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
160000 | - |
gel filtration | Lepidium latifolium |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Lepidium latifolium | - |
- |
- |
Purification (Comment) | Organism |
---|---|
native enzyme 69.3fold from leaves to homogeneity by ammonium sulfate fractionation, ultrafiltration, concanavalin A affinity chromatography, and gel filtation | Lepidium latifolium |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Lepidium latifolium | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
3.12 | - |
purified native enzyme, pH 6.0, 37°C, substrate sinigrin | Lepidium latifolium |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
sinigrin + H2O | - |
Lepidium latifolium | (1Z)-N-(sulfooxy)but-3-enimidothioic acid + D-glucose | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 70000, about, SDS-PAGE | Lepidium latifolium |
Synonyms | Comment | Organism |
---|---|---|
beta-thioglucoside glucohydrolase | - |
Lepidium latifolium |
myrosinase | - |
Lepidium latifolium |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
- |
Lepidium latifolium |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
4 | 90 | activity range, profile overview. Over 50% of maximal activity at 30-60°C | Lepidium latifolium |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
95 | - |
purified native enzyme, 10 min, pH 6.0, inactivation | Lepidium latifolium |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.33 | - |
sinigrin | pH 6.0, 37°C, without GSH | Lepidium latifolium | |
0.75 | - |
sinigrin | pH 6.0, 37°C, with 1 mM GSH | Lepidium latifolium |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
- |
Lepidium latifolium |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
3 | 10.7 | activity range, profile overview | Lepidium latifolium |
General Information | Comment | Organism |
---|---|---|
evolution | the purified enzyme from Lepidum latifolium is encoded by the MYR I gene subfamily | Lepidium latifolium |
physiological function | the enzyme is a redox-regulated isoform of myrosinase. Myrosinase is involved in the hydrolysis of glucosinolates to isothiocyanates, nitriles, and thiocyanates that are responsible for various ecological and health benefits. The thiol-regulated kinetic behavior of the myrosinase isozyme from Lepidium latifolium signifies the enzyme's strategy to fine-tune its activity in different redox environments, thus regulating its biological effects. A responsive glucosinolate-myrosinase system in this plant seems possible owing to its high glucosinolate content | Lepidium latifolium |