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Literature summary for 3.2.1.147 extracted from

  • Bhat, R.; Kaur, T.; Khajuria, M.; Vyas, R.; Vyas, D.
    Purification and characterization of a novel redox-regulated isoform of myrosinase (beta-thioglucoside glucohydrolase) from Lepidium latifolium L. (2015), J. Agric. Food Chem., 63, 10218-10226 .
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
ascorbate activates, best at 0.7 mM Lepidium latifolium
DTT activates at 0.5-5 mM Lepidium latifolium
EDTA
-
Lepidium latifolium
glutathione GSH, reduced forms of the enzyme are more active Lepidium latifolium
Urea
-
Lepidium latifolium

Inhibitors

Inhibitors Comment Organism Structure
Ba2+
-
Lepidium latifolium
Ca2+
-
Lepidium latifolium
Cu2+ 25% inhibition at 10 mM Lepidium latifolium
D-glucose
-
Lepidium latifolium
Mg2+
-
Lepidium latifolium
Mn2+
-
Lepidium latifolium
SDS
-
Lepidium latifolium
Zn2+
-
Lepidium latifolium

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.162
-
sinigrin pH 6.0, 37°C, with 1 mM GSH Lepidium latifolium
0.171
-
sinigrin pH 6.0, 37°C, without GSH Lepidium latifolium

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
160000
-
gel filtration Lepidium latifolium

Organism

Organism UniProt Comment Textmining
Lepidium latifolium
-
-
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme 69.3fold from leaves to homogeneity by ammonium sulfate fractionation, ultrafiltration, concanavalin A affinity chromatography, and gel filtation Lepidium latifolium

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
Lepidium latifolium
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.12
-
purified native enzyme, pH 6.0, 37°C, substrate sinigrin Lepidium latifolium

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
sinigrin + H2O
-
Lepidium latifolium (1Z)-N-(sulfooxy)but-3-enimidothioic acid + D-glucose
-
?

Subunits

Subunits Comment Organism
dimer 2 * 70000, about, SDS-PAGE Lepidium latifolium

Synonyms

Synonyms Comment Organism
beta-thioglucoside glucohydrolase
-
Lepidium latifolium
myrosinase
-
Lepidium latifolium

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
Lepidium latifolium

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
4 90 activity range, profile overview. Over 50% of maximal activity at 30-60°C Lepidium latifolium

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
95
-
purified native enzyme, 10 min, pH 6.0, inactivation Lepidium latifolium

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.33
-
sinigrin pH 6.0, 37°C, without GSH Lepidium latifolium
0.75
-
sinigrin pH 6.0, 37°C, with 1 mM GSH Lepidium latifolium

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
Lepidium latifolium

pH Range

pH Minimum pH Maximum Comment Organism
3 10.7 activity range, profile overview Lepidium latifolium

General Information

General Information Comment Organism
evolution the purified enzyme from Lepidum latifolium is encoded by the MYR I gene subfamily Lepidium latifolium
physiological function the enzyme is a redox-regulated isoform of myrosinase. Myrosinase is involved in the hydrolysis of glucosinolates to isothiocyanates, nitriles, and thiocyanates that are responsible for various ecological and health benefits. The thiol-regulated kinetic behavior of the myrosinase isozyme from Lepidium latifolium signifies the enzyme's strategy to fine-tune its activity in different redox environments, thus regulating its biological effects. A responsive glucosinolate-myrosinase system in this plant seems possible owing to its high glucosinolate content Lepidium latifolium