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Literature summary for 3.2.1.147 extracted from

  • Dosz, E.B.; Ku, K.M.; Juvik, J.A.; Jeffery, E.H.
    Total myrosinase activity estimates in brassica vegetable produce (2014), J. Agric. Food Chem., 62, 8094-8100.
    View publication on PubMed

Organism

Organism UniProt Comment Textmining
Brassica oleracea
-
broccoli, cv. Sultan, Marathon, Patron, Pirate, Maximo
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Brassica oleracea
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kale, Acephala Group, cv. Red Winter, Red Russian, Dwarf Blue Curled Vates
-

Source Tissue

Source Tissue Comment Organism Textmining
floret
-
Brassica oleracea
-
leaf
-
Brassica oleracea
-
root
-
Brassica oleracea
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.69 2.04 enzyme from florets of the different cultivars, pH not specified in the publication, glucose assay, 40°C Brassica oleracea
0.73 0.86 enzyme from roots of the different cultivars, pH not specified in the publication, glucose assay, 40°C Brassica oleracea
1.09 1.68 enzyme from roots of the different cultivars, pH not specified in the publication, glucose assay, 40°C Brassica oleracea
1.27 1.85 enzyme from leaves of the different cultivars, pH not specified in the publication, glucose assay, 40°C Brassica oleracea
1.96 2.39 enzyme from leaves of the different cultivars, pH not specified in the publication, glucose assay, 40°C Brassica oleracea
4.44 10.68 enzyme from roots of the different cultivars, pH not specified in the publication, AITC assay, 25°C Brassica oleracea
6.37 15.3 enzyme from roots of the different cultivars, pH not specified in the publication, AITC assay, 25°C Brassica oleracea
6.87 14.7 enzyme from leaves of the different cultivars, pH not specified in the publication, AITC assay, 25°C Brassica oleracea
13.08 15.62 enzyme from florets of the different cultivars, pH not specified in the publication, AITC assay, 25°C Brassica oleracea
14.46 20.6 enzyme from leaves of the different cultivars, pH not specified in the publication, AITC assay, 25°C Brassica oleracea

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
sinigrin + H2O
-
Brassica oleracea ?
-
?

Synonyms

Synonyms Comment Organism
myrosinase
-
Brassica oleracea

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25 40 assay at Brassica oleracea