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Literature summary for 3.2.1.147 extracted from

  • Ahuja, I.; Borgen, B.H.; Hansen, M.; Honne, B.I.; Mueller, C.; Rohloff, J.; Rossiter, J.T.; Bones, A.M.
    Oilseed rape seeds with ablated defence cells of the glucosinolate-myrosinase system. Production and characteristics of double haploid MINELESS plants of Brassica napus L. (2011), J. Exp. Bot., 62, 4975-4993.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.2.1.147

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
65000
-
 x * 65000, SDS-PAGE. Immunoblot analysis reveals the major bands of myrosinase polypeptide classes (denoted as 65, 70, and 75 kDa) in wild type samples of seeds Brassica napus
70000
-
 x * 70000, SDS-PAGE. Immunoblot analysis reveals the major bands of myrosinase polypeptide classes (denoted as 65, 70, and 75 kDa) in wild type samples of seeds Brassica napus
75000
-
 x * 75000, SDS-PAGE. Immunoblot analysis reveals the major bands of myrosinase polypeptide classes (denoted as 65, 70, and 75 kDa) in wild type samples of seeds Brassica napus

Organism

Organism UniProt Comment Textmining
Brassica napus
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
myrosin cell
-
 Brassica napus
-
seed
-
 Brassica napus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 the specific myrosinase activity in wild type seeds ranges from 0.1186-0.4263 micromol glucose/min/mg protein Brassica napus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
sinigrin + H2O
-
 Brassica napus D-glucose + isothiocyanate + ?
-
 ?

Subunits

Subunits Comment Organism
? x * 65000, SDS-PAGE. Immunoblot analysis reveals the major bands of myrosinase polypeptide classes (denoted as 65, 70, and 75 kDa) in wild type samples of seeds Brassica napus
? x * 70000, SDS-PAGE. Immunoblot analysis reveals the major bands of myrosinase polypeptide classes (denoted as 65, 70, and 75 kDa) in wild type samples of seeds Brassica napus
? x * 75000, SDS-PAGE. Immunoblot analysis reveals the major bands of myrosinase polypeptide classes (denoted as 65, 70, and 75 kDa) in wild type samples of seeds Brassica napus

Synonyms

Synonyms Comment Organism
myrosinase
-
 Brassica napus

General Information

General Information Comment Organism
malfunction double haploid myrosin cell-free plants (MINELESS plants) have significantly reduced myrosinase levels and glucosinolate hydrolysis products Brassica napus
physiological function Brassica napus contains a defence system known as the glucosinolate-myrosinase system or the mustard oil bomb. The mustard oil bomb which includes myrosinase and glucosinolates is triggered by abiotic and biotic stress, resulting in the formation of toxic products such as nitriles and isothiocyanates Brassica napus