Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.2.1.147 extracted from

  • Besle, A.; Brazzolotto, X.; Tatibouet, A.; Cerniauskaite, D.; Gallienne, E.; Rollin, P.; Burmeister, W.P.
    A micromolar O-sulfated thiohydroximate inhibitor bound to plant myrosinase (2010), Acta Crystallogr. Sect. F, 66, 152-155.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.2.1.147

Crystallization (Commentary)

Crystallization (Comment) Organism
purified enzyme in 20 mM HEPES, pH 6.5, 150 mM NaCl, and 0.02 mM ZnSO4, X-ray diffraction structure determination and analysis at 1.6 A resolution Sinapis alba

Inhibitors

Inhibitors Comment Organism Structure
(Z)-(1-((2-(dimethylammonio)ethyl)thio)-2-phenylethylidene)amino sulfate a competitive inhibitor. The sulfate group and the phenyl group of the inhibitor bind to the aglycon-binding site of the enzyme, whereas the N,N-dimethyl group binds to the glucose-binding site, binding structure, overview Sinapis alba

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Sinapis alba myrosinase is a unique enzyme which catalyzes the hydrolysis of sulfur-containing secondary metabolites called glucosinolates ?
-
 ?

Organism

Organism UniProt Comment Textmining
Sinapis alba P29736
-
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme from seeds Sinapis alba

Source Tissue

Source Tissue Comment Organism Textmining
seed
-
 Sinapis alba
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information myrosinase is a unique enzyme which catalyzes the hydrolysis of sulfur-containing secondary metabolites called glucosinolates Sinapis alba ?
-
 ?

Synonyms

Synonyms Comment Organism
myrosinase
-
 Sinapis alba

General Information

General Information Comment Organism
physiological function myrosinase and its substrates, the glucosinolates, are part of the plant's defense system Sinapis alba