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Literature summary for 3.2.1.147 extracted from
- Studies on myrosinases. III. Enzymatic properties of myrosinases from Sinapis alba and Brassica napus seeds (1973), Biochim. Biophys. Acta, 327, 121-131.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| ascorbic acid | - |
Brassica napus |  |
| ascorbic acid | 1 mM, activation of isoenzyme RA, RB and RC | Sinapis alba | |
| ascorbic acid | 1 mM, activation of isoenzyme SA, SB, very low activation of isoenzyme SC | Sinapis alba | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.03 | - |
sinigrin | isoenzyme RC, without ascorbic acid | Brassica napus | |
| 0.05 | - |
sinigrin | isoenzyme RA, without ascorbic acid | Brassica napus | |
| 0.06 | - |
sinigrin | isoenzyme RB, without ascorbic acid | Brassica napus | |
| 0.06 | - |
sinigrin | isoenzyme SB, without ascorbic acid | Sinapis alba | |
| 0.17 | - |
sinigrin | isoenzyme SC, without ascorbic acid | Sinapis alba | |
| 0.25 | - |
sinigrin | isoenzyme SA, in presence of 1 mM ascorbic acid | Sinapis alba | |
| 0.3 | - |
sinigrin | isoenzyme RC in presence of 1 mM ascorbic acid | Brassica napus | |
| 0.3 | - |
sinigrin | isoenzyme SB, in presence of 1 mM ascorbic acid | Sinapis alba | |
| 0.4 | - |
sinigrin | isoenzyme RA and RB, in presence of 1 mM ascorbic acid | Brassica napus | |
| 0.4 | - |
sinigrin | isoenzyme SC, in presence of 1 mM ascorbic acid | Sinapis alba | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Brassica napus | - |
4 isoenzymes: RA, RB, RC and RD | - |
| Sinapis alba | - |
3 isoenzymes: SA, SB and SC | - |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| seed | - |
Brassica napus | - |
| seed | - |
Sinapis alba | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| glucoapparin + H2O | - |
Brassica napus | ? | - |
? | |
| glucoapparin + H2O | - |
Sinapis alba | ? | - |
? | |
| glucocheirolin + H2O | - |
Brassica napus | ? | - |
? | |
| glucocheirolin + H2O | - |
Sinapis alba | ? | - |
? | |
| glucosinalbin + H2O | - |
Brassica napus | ? | - |
? | |
| glucosinalbin + H2O | - |
Sinapis alba | ? | - |
? | |
| glucotropaeolin + H2O | - |
Brassica napus | ? | - |
? | |
| glucotropaeolin + H2O | - |
Sinapis alba | ? | - |
? | |
| progoitrin + H2O | - |
Brassica napus | additional information | - |
? | |
| progoitrin + H2O | - |
Sinapis alba | additional information | - |
? | |
| sinigrin + H2O | - |
Brassica napus | D-glucose + 3-isothiocyanatoprop-1-ene + SO42- | - |
? | |
| sinigrin + H2O | - |
Sinapis alba | D-glucose + 3-isothiocyanatoprop-1-ene + SO42- | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
isoenzyme SA, SB and SC | Sinapis alba |
| 60 | - |
isoenzyme RA, RB, RC and RD | Brassica napus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4 | - |
isoenzyme SA | Sinapis alba |
| 4.3 | - |
isoenzyme RC | Brassica napus |
| 4.5 | 5.5 | isoenzyme SB | Sinapis alba |
| 5 | - |
isoenzyme RA and RB | Brassica napus |
| 5.5 | - |
isoenzyme SC | Sinapis alba |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
maximal stability | Brassica napus |
| 6 | - |
maximal stability | Sinapis alba |
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