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Literature summary for 3.2.1.147 extracted from

  • Petroski, R.J.
    Stereoselectivity of the interaction of thioglucoside glucohydrolase and epithiospecifier protein from various sources (1986), Plant Sci., 44, 85-88.
No PubMed abstract available
Search for more literature for 3.2.1.147

Activating Compound

Activating Compound Comment Organism Structure
epithiospecifier protein ESP, is a small protein of molecular weight 30 to 40 kDa, which co-occurs with myrosinase. ESP does not have thioglucosidase activity, but interacts with myrosinase to promote the transfer of sulfur from the S-glucose moiety of terminally unsaturated glucosinolates to the alkenyl moiety, resulting in the formation of epithionitriles. The presence of ferrous ions are essential for ESP function. Aspergillus sydowii
epithiospecifier protein ESP, is a small protein of molecular weight 30 to 40 kDa, which co-occurs with myrosinase. ESP does not have thioglucosidase activity, but interacts with myrosinase to promote the transfer of sulfur from the S-glucose moiety of terminally unsaturated glucosinolates to the alkenyl moiety, resulting in the formation of epithionitriles. The presence of ferrous ions are essential for ESP function. Brassica rapa
epithiospecifier protein ESP, is a small protein of molecular weight 30 to 40 kDa, which co-occurs with myrosinase. ESP does not have thioglucosidase activity, but interacts with myrosinase to promote the transfer of sulfur from the S-glucose moiety of terminally unsaturated glucosinolates to the alkenyl moiety, resulting in the formation of epithionitriles. The presence of ferrous ions are essential for ESP function. Sinapis alba
epithiospecifier protein ESP, is a small protein of molecular weight 30 to 40 kDa, which co-occurs with myrosinase. ESP does not have thioglucosidase activity, but interacts with myrosinase to promote the transfer of sulfur from the S-glucose moiety of terminally unsaturated glucosinolates to the alkenyl moiety, resulting in the formation of epithionitriles. The presence of ferrous ions are essential for ESP function. Crambe hispanica subsp. abyssinica
epithiospecifier protein a mixture of products which includes 1-cyano-2-hydroxy-3-butene, (R)-5-vinyloxazolidine-2-thione, D-glucose, HSO4- and elemental sulfur is formed from (S)-2-hydroxy-3-butenylglucosinolate without epithiospecifier protein at pH 5.9. These products as well as erythro- and threo-1-cyano-2-hydroxy-3,4-epithiobutanes are formed by combination of the enzyme and epithiospecifier protein from various sources Sinapis alba

Organism

Organism UniProt Comment Textmining
Aspergillus sydowii
-
-
-
Brassica rapa
-
 ssp. rapifera
-
Crambe hispanica subsp. abyssinica
-
-
-
Sinapis alba
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-hydroxybut-3-enylglucosinolate + H2O (S)-2-hydroxy-3-butenylglucosinolate, i.e. epi-progoitrin Sinapis alba additional information a mixture of products which includes 1-cyano-2-hydroxy-3-butene, (R)-5-vinyloxazolidine-2-thione, D-glucose, HSO4- and elemental sulfur is formed without epithiospecifier protein at pH 5.9. These products as well as erythro-1-cyano-2-hydroxy-3,4-epithiobutanes and threo-1-cyano-2-hydroxy-3,4-epithiobutanes are formed by combination of the enzyme and epithiospecifier protein from various sources ?
sinigrin + H2O
-
 Aspergillus sydowii D-glucose + 3-isothiocyanatoprop-1-ene + SO42-
-
 ?
sinigrin + H2O
-
 Brassica rapa D-glucose + 3-isothiocyanatoprop-1-ene + SO42-
-
 ?
sinigrin + H2O
-
 Sinapis alba D-glucose + 3-isothiocyanatoprop-1-ene + SO42-
-
 ?
sinigrin + H2O
-
 Crambe hispanica subsp. abyssinica D-glucose + 3-isothiocyanatoprop-1-ene + SO42-
-
 ?