Cloned (Comment) | Organism |
---|---|
orf0232, DNA and amino acid sequence determination and analysis, recombinant expression of N-terminally 2 x His6- and Nus-tagged enzyme in Escherichia coli strain BL21(DE3)CodonPlus | Streptomyces viridochromogenes |
orf1110, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic tree, recombinant expression of N-terminally 2 x His6- and Nus-tagged enzyme in Escherichia coli strain BL21(DE3)CodonPlus | Streptomyces sp. JHA19 |
orf2125, DNA and amino acid sequence determination and analysis, recombinant expression of N-termially 2 x His6- and Nus-tagged enzyme in Escherichia coli strain BL21(DE3)CodonPlus | Streptomyces chartreusis |
orf2812, DNA and amino acid sequence determination and analysis, recombinant expression of N-termially 2 x His6- and Nus-tagged enzyme in Escherichia coli strain BL21(DE3)CodonPlus | Streptomyces sp. |
Protein Variants | Comment | Organism |
---|---|---|
D183A | site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme | Streptomyces sp. JHA19 |
D201A | site-directed mutagenesis, the mutant shows highly reduced activity compared to the wild-type enzyme | Streptomyces sp. JHA19 |
D330A | site-directed mutagenesis, the mutant shows highly reduced activity compared to the wild-type enzyme | Streptomyces sp. JHA19 |
D336A | site-directed mutagenesis, the mutant shows equal activity as the wild-type enzyme | Streptomyces sp. JHA19 |
D372A | site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme | Streptomyces sp. JHA19 |
D386A | site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme | Streptomyces sp. JHA19 |
D414A | site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme | Streptomyces sp. JHA19 |
D423A | site-directed mutagenesis, the mutant shows highly reduced activity compared to the wild-type enzyme | Streptomyces sp. JHA19 |
D482A | site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme | Streptomyces sp. JHA19 |
D500A | site-directed mutagenesis, the mutant shows highly reduced activity compared to the wild-type enzyme | Streptomyces sp. JHA19 |
D508A | site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme | Streptomyces sp. JHA19 |
D590A | site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme | Streptomyces sp. JHA19 |
D602A | site-directed mutagenesis, the mutant shows slightly increased activity compared to the wild-type enzyme | Streptomyces sp. JHA19 |
E405A | site-directed mutagenesis, the mutant shows highly reduced activity compared to the wild-type enzyme | Streptomyces sp. JHA19 |
E464A | site-directed mutagenesis, the mutant shows highly reduced activity compared to the wild-type enzyme | Streptomyces sp. JHA19 |
E530A | site-directed mutagenesis, the mutant shows highly reduced activity compared to the wild-type enzyme | Streptomyces sp. JHA19 |
E592A | site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme | Streptomyces sp. JHA19 |
E594A | site-directed mutagenesis, the mutant shows highly reduced activity compared to the wild-type enzyme | Streptomyces sp. JHA19 |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
4.4 | - |
4-nitrophenyl beta-D-galactofuranoside | pH 5.5, 37°C, recombinant enzyme | Streptomyces sp. JHA19 |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces chartreusis | A0A0K2SQX3 | isolated from soil in Kagawa University, Japan | - |
Streptomyces chartreusis JHA19 | A0A0K2SQX3 | isolated from soil in Kagawa University, Japan | - |
Streptomyces sp. | A0A0K2SQZ9 | isolated from soil in Kagawa University, Japan | - |
Streptomyces sp. JHA19 | A0A0K2SRV3 | isolated from soil in Kagawa University, Japan | - |
Streptomyces sp. JHA19 NRRL F-5140 | A0A0K2SRV3 | isolated from soil in Kagawa University, Japan | - |
Streptomyces sp. NRRL F-5140 | A0A0K2SQZ9 | isolated from soil in Kagawa University, Japan | - |
Streptomyces viridochromogenes | A0A0K2SQX4 | isolated from soil in Kagawa University, Japan | - |
Streptomyces viridochromogenes JHA19 | A0A0K2SQX4 | isolated from soil in Kagawa University, Japan | - |
Purification (Comment) | Organism |
---|---|
recombinant N-termially 2 x His6- and Nus-tagged enzyme from Escherichia coli strain BL21(DE3)CodonPlus, by nickel affinity chromatography, tag cleavage by HRV3C protease, followed by nickel affinity chromatography and ultrafiltration | Streptomyces sp. |
recombinant N-termially 2 x His6- and Nus-tagged enzyme from Escherichia coli strain BL21(DE3)CodonPlus, by nickel affinity chromatography, tag cleavage by HRV3C protease, followed by nickel affinity chromatography and ultrafiltration | Streptomyces chartreusis |
recombinant N-terminally 2 x His6- and Nus-tagged enzyme from Escherichia coli strain BL21(DE3)CodonPlus, by nickel affinity chromatography, tag cleavage by HRV3C protease, followed by nickel affinity chromatography and ultrafiltration | Streptomyces sp. JHA19 |
recombinant N-terminally 2 x His6- and Nus-tagged enzyme from Escherichia coli strain BL21(DE3)CodonPlus, by nickel affinity chromatography, tag cleavage by HRV3C protease, followed by nickel affinity chromatography and ultrafiltration | Streptomyces viridochromogenes |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl beta-D-galactofuranoside + H2O | - |
Streptomyces sp. | 4-nitrophenol + D-galactofuranose | - |
? | |
4-nitrophenyl beta-D-galactofuranoside + H2O | - |
Streptomyces chartreusis | 4-nitrophenol + D-galactofuranose | - |
? | |
4-nitrophenyl beta-D-galactofuranoside + H2O | - |
Streptomyces sp. JHA19 | 4-nitrophenol + D-galactofuranose | - |
? | |
4-nitrophenyl beta-D-galactofuranoside + H2O | - |
Streptomyces viridochromogenes | 4-nitrophenol + D-galactofuranose | - |
? | |
4-nitrophenyl beta-D-galactofuranoside + H2O | - |
Streptomyces chartreusis JHA19 | 4-nitrophenol + D-galactofuranose | - |
? | |
4-nitrophenyl beta-D-galactofuranoside + H2O | - |
Streptomyces sp. JHA19 NRRL F-5140 | 4-nitrophenol + D-galactofuranose | - |
? | |
4-nitrophenyl beta-D-galactofuranoside + H2O | - |
Streptomyces viridochromogenes JHA19 | 4-nitrophenol + D-galactofuranose | - |
? | |
4-nitrophenyl beta-D-galactofuranoside + H2O | - |
Streptomyces sp. NRRL F-5140 | 4-nitrophenol + D-galactofuranose | - |
? | |
galactomannan + H2O | release of galactose residue, galactomannan prepared from Aspergillus fumigatus | Streptomyces sp. | ? | - |
? | |
galactomannan + H2O | release of galactose residue, galactomannan prepared from Aspergillus fumigatus | Streptomyces chartreusis | ? | - |
? | |
galactomannan + H2O | release of galactose residue, galactomannan prepared from Aspergillus fumigatus | Streptomyces sp. JHA19 | ? | - |
? | |
galactomannan + H2O | release of galactose residue, galactomannan prepared from Aspergillus fumigatus | Streptomyces viridochromogenes | ? | - |
? | |
galactomannan + H2O | release of galactose residue, galactomannan prepared from Aspergillus fumigatus | Streptomyces chartreusis JHA19 | ? | - |
? | |
galactomannan + H2O | release of galactose residue, galactomannan prepared from Aspergillus fumigatus | Streptomyces sp. JHA19 NRRL F-5140 | ? | - |
? | |
galactomannan + H2O | release of galactose residue, galactomannan prepared from Aspergillus fumigatus | Streptomyces viridochromogenes JHA19 | ? | - |
? | |
galactomannan + H2O | release of galactose residue, galactomannan prepared from Aspergillus fumigatus | Streptomyces sp. NRRL F-5140 | ? | - |
? | |
additional information | four genes in Streptomyces sp. JHA19 encode Galf-ases. One enzyme (beta-D-galactofuranosidase 2) is a Galf-specific hydrolase, that shows no activity with alpha-L-arabinofuranosides, while the other three (beta-D-galactofuranosidases 1, 3, and 4) are active as alpha-L-arabinofuranosidases (Araf-ases, EC 3.2.1.55) and also as beta-D-galactofuranosidases | Streptomyces sp. | ? | - |
? | |
additional information | four genes in Streptomyces sp. JHA19 encode Galf-ases. One enzyme (beta-D-galactofuranosidase 2) is a Galf-specific hydrolase, that shows no activity with alpha-L-arabinofuranosides, while the other three (beta-D-galactofuranosidases 1, 3, and 4) are active as alpha-L-arabinofuranosidases (Araf-ases, EC 3.2.1.55) and also as beta-D-galactofuranosidases | Streptomyces chartreusis | ? | - |
? | |
additional information | four genes in Streptomyces sp. JHA19 encode Galf-ases. One enzyme (beta-D-galactofuranosidase 2) is a Galf-specific hydrolase, that shows no activity with alpha-L-arabinofuranosides, while the other three (beta-D-galactofuranosidases 1, 3, and 4) are active as alpha-L-arabinofuranosidases (Araf-ases, EC 3.2.1.55) and also as beta-D-galactofuranosidases | Streptomyces viridochromogenes | ? | - |
? | |
additional information | four genes in Streptomyces sp. JHA19 encode Galf-ases. The beta-D-galactofuranosidase 2 enzyme is a Galf-specific hydrolase, that shows no activity with alpha-L-arabinofuranosides, while the other three are also active as alpha-L-arabinofuranosidases (Araf-ases, EC 3.2.1.55) | Streptomyces sp. JHA19 | ? | - |
? | |
additional information | four genes in Streptomyces sp. JHA19 encode Galf-ases. One enzyme (beta-D-galactofuranosidase 2) is a Galf-specific hydrolase, that shows no activity with alpha-L-arabinofuranosides, while the other three (beta-D-galactofuranosidases 1, 3, and 4) are active as alpha-L-arabinofuranosidases (Araf-ases, EC 3.2.1.55) and also as beta-D-galactofuranosidases | Streptomyces chartreusis JHA19 | ? | - |
? | |
additional information | four genes in Streptomyces sp. JHA19 encode Galf-ases. The beta-D-galactofuranosidase 2 enzyme is a Galf-specific hydrolase, that shows no activity with alpha-L-arabinofuranosides, while the other three are also active as alpha-L-arabinofuranosidases (Araf-ases, EC 3.2.1.55) | Streptomyces sp. JHA19 NRRL F-5140 | ? | - |
? | |
additional information | four genes in Streptomyces sp. JHA19 encode Galf-ases. One enzyme (beta-D-galactofuranosidase 2) is a Galf-specific hydrolase, that shows no activity with alpha-L-arabinofuranosides, while the other three (beta-D-galactofuranosidases 1, 3, and 4) are active as alpha-L-arabinofuranosidases (Araf-ases, EC 3.2.1.55) and also as beta-D-galactofuranosidases | Streptomyces viridochromogenes JHA19 | ? | - |
? | |
additional information | four genes in Streptomyces sp. JHA19 encode Galf-ases. One enzyme (beta-D-galactofuranosidase 2) is a Galf-specific hydrolase, that shows no activity with alpha-L-arabinofuranosides, while the other three (beta-D-galactofuranosidases 1, 3, and 4) are active as alpha-L-arabinofuranosidases (Araf-ases, EC 3.2.1.55) and also as beta-D-galactofuranosidases | Streptomyces sp. NRRL F-5140 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Araf-ase | - |
Streptomyces sp. |
Araf-ase | - |
Streptomyces chartreusis |
Araf-ase | - |
Streptomyces viridochromogenes |
beta-D-galactofuranosidase | - |
Streptomyces sp. JHA19 |
beta-D-galactofuranosidase 1 | UniProt | Streptomyces viridochromogenes |
beta-D-galactofuranosidase 2 | - |
Streptomyces sp. JHA19 |
beta-D-galactofuranosidase 3 | UniProt | Streptomyces chartreusis |
beta-D-galactofuranosidase 4 | UniProt | Streptomyces sp. |
galactofuranose-specific beta-D-galactofuranosidase | - |
Streptomyces sp. JHA19 |
Galf-ase | - |
Streptomyces sp. |
Galf-ase | - |
Streptomyces chartreusis |
Galf-ase | - |
Streptomyces sp. JHA19 |
Galf-ase | - |
Streptomyces viridochromogenes |
Galf-specific Galf-ase | - |
Streptomyces sp. JHA19 |
Galf-specific hydrolase | - |
Streptomyces sp. JHA19 |
More | see also EC 3.2.1.55 | Streptomyces sp. |
More | see also EC 3.2.1.55 | Streptomyces chartreusis |
More | see also EC 3.2.1.55 | Streptomyces viridochromogenes |
ORF0232 | locus name | Streptomyces viridochromogenes |
ORF1110 | locus name | Streptomyces sp. JHA19 |
ORF2125 | locus name | Streptomyces chartreusis |
ORF2812 | locus name | Streptomyces sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Streptomyces sp. |
37 | - |
assay at | Streptomyces chartreusis |
37 | - |
assay at | Streptomyces sp. JHA19 |
37 | - |
assay at | Streptomyces viridochromogenes |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
purified recombinant detagged enzyme, pH 5.5, 10 min, stable up to | Streptomyces sp. JHA19 |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.5 | - |
recombinant enzyme | Streptomyces sp. JHA19 |
5.5 | - |
assay at | Streptomyces sp. |
5.5 | - |
assay at | Streptomyces chartreusis |
5.5 | - |
assay at | Streptomyces viridochromogenes |
General Information | Comment | Organism |
---|---|---|
evolution | four genes in Streptomyces sp. JHA19 encode beta-D-galactofuranosidase (Galf-ases). One enzyme (beta-D-galactofuranosidase 2) is a Galf-specific hydrolase, that shows no activity with alpha-L-arabinofuranosides, while the other three (beta-D-galactofuranosidases 1, 3, and 4) are also active as alpha-L-arabinofuranosidases (Araf-ases, EC 3.2.1.55) | Streptomyces sp. |
evolution | four genes in Streptomyces sp. JHA19 encode Galf-ases. One enzyme (beta-D-galactofuranosidase 2) is a Galf-specific hydrolase, that shows no activity with alpha-L-arabinofuranosides, while the other three (beta-D-galactofuranosidases 1, 3, and 4) are also active as alpha-L-arabinofuranosidases (Araf-ases, EC 3.2.1.55) | Streptomyces chartreusis |
evolution | four genes in Streptomyces sp. JHA19 encode Galf-ases. One enzyme (beta-D-galactofuranosidase 2) is a Galf-specific hydrolase, that shows no activity with alpha-L-arabinofuranosides, while the other three (beta-D-galactofuranosidases 1, 3, and 4) are also active as alpha-L-arabinofuranosidases (Araf-ases, EC 3.2.1.55) | Streptomyces viridochromogenes |