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Literature summary for 3.2.1.146 extracted from

  • Matsunaga, E.; Higuchi, Y.; Mori, K.; Yairo, N.; Oka, T.; Shinozuka, S.; Tashiro, K.; Izumi, M.; Kuhara, S.; Takegawa, K.
    Identification and characterization of a novel galactofuranose-specific beta-D-galactofuranosidase from Streptomyces species (2015), PLoS ONE, 10, e0137230 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
orf0232, DNA and amino acid sequence determination and analysis, recombinant expression of N-terminally 2 x His6- and Nus-tagged enzyme in Escherichia coli strain BL21(DE3)CodonPlus Streptomyces viridochromogenes
orf1110, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic tree, recombinant expression of N-terminally 2 x His6- and Nus-tagged enzyme in Escherichia coli strain BL21(DE3)CodonPlus Streptomyces sp. JHA19
orf2125, DNA and amino acid sequence determination and analysis, recombinant expression of N-termially 2 x His6- and Nus-tagged enzyme in Escherichia coli strain BL21(DE3)CodonPlus Streptomyces chartreusis
orf2812, DNA and amino acid sequence determination and analysis, recombinant expression of N-termially 2 x His6- and Nus-tagged enzyme in Escherichia coli strain BL21(DE3)CodonPlus Streptomyces sp.

Protein Variants

Protein Variants Comment Organism
D183A site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme Streptomyces sp. JHA19
D201A site-directed mutagenesis, the mutant shows highly reduced activity compared to the wild-type enzyme Streptomyces sp. JHA19
D330A site-directed mutagenesis, the mutant shows highly reduced activity compared to the wild-type enzyme Streptomyces sp. JHA19
D336A site-directed mutagenesis, the mutant shows equal activity as the wild-type enzyme Streptomyces sp. JHA19
D372A site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme Streptomyces sp. JHA19
D386A site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme Streptomyces sp. JHA19
D414A site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme Streptomyces sp. JHA19
D423A site-directed mutagenesis, the mutant shows highly reduced activity compared to the wild-type enzyme Streptomyces sp. JHA19
D482A site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme Streptomyces sp. JHA19
D500A site-directed mutagenesis, the mutant shows highly reduced activity compared to the wild-type enzyme Streptomyces sp. JHA19
D508A site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme Streptomyces sp. JHA19
D590A site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme Streptomyces sp. JHA19
D602A site-directed mutagenesis, the mutant shows slightly increased activity compared to the wild-type enzyme Streptomyces sp. JHA19
E405A site-directed mutagenesis, the mutant shows highly reduced activity compared to the wild-type enzyme Streptomyces sp. JHA19
E464A site-directed mutagenesis, the mutant shows highly reduced activity compared to the wild-type enzyme Streptomyces sp. JHA19
E530A site-directed mutagenesis, the mutant shows highly reduced activity compared to the wild-type enzyme Streptomyces sp. JHA19
E592A site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme Streptomyces sp. JHA19
E594A site-directed mutagenesis, the mutant shows highly reduced activity compared to the wild-type enzyme Streptomyces sp. JHA19

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.4
-
4-nitrophenyl beta-D-galactofuranoside pH 5.5, 37°C, recombinant enzyme Streptomyces sp. JHA19

Organism

Organism UniProt Comment Textmining
Streptomyces chartreusis A0A0K2SQX3 isolated from soil in Kagawa University, Japan
-
Streptomyces chartreusis JHA19 A0A0K2SQX3 isolated from soil in Kagawa University, Japan
-
Streptomyces sp. A0A0K2SQZ9 isolated from soil in Kagawa University, Japan
-
Streptomyces sp. JHA19 A0A0K2SRV3 isolated from soil in Kagawa University, Japan
-
Streptomyces sp. JHA19 NRRL F-5140 A0A0K2SRV3 isolated from soil in Kagawa University, Japan
-
Streptomyces sp. NRRL F-5140 A0A0K2SQZ9 isolated from soil in Kagawa University, Japan
-
Streptomyces viridochromogenes A0A0K2SQX4 isolated from soil in Kagawa University, Japan
-
Streptomyces viridochromogenes JHA19 A0A0K2SQX4 isolated from soil in Kagawa University, Japan
-

Purification (Commentary)

Purification (Comment) Organism
recombinant N-termially 2 x His6- and Nus-tagged enzyme from Escherichia coli strain BL21(DE3)CodonPlus, by nickel affinity chromatography, tag cleavage by HRV3C protease, followed by nickel affinity chromatography and ultrafiltration Streptomyces sp.
recombinant N-termially 2 x His6- and Nus-tagged enzyme from Escherichia coli strain BL21(DE3)CodonPlus, by nickel affinity chromatography, tag cleavage by HRV3C protease, followed by nickel affinity chromatography and ultrafiltration Streptomyces chartreusis
recombinant N-terminally 2 x His6- and Nus-tagged enzyme from Escherichia coli strain BL21(DE3)CodonPlus, by nickel affinity chromatography, tag cleavage by HRV3C protease, followed by nickel affinity chromatography and ultrafiltration Streptomyces sp. JHA19
recombinant N-terminally 2 x His6- and Nus-tagged enzyme from Escherichia coli strain BL21(DE3)CodonPlus, by nickel affinity chromatography, tag cleavage by HRV3C protease, followed by nickel affinity chromatography and ultrafiltration Streptomyces viridochromogenes

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl beta-D-galactofuranoside + H2O
-
Streptomyces sp. 4-nitrophenol + D-galactofuranose
-
?
4-nitrophenyl beta-D-galactofuranoside + H2O
-
Streptomyces chartreusis 4-nitrophenol + D-galactofuranose
-
?
4-nitrophenyl beta-D-galactofuranoside + H2O
-
Streptomyces sp. JHA19 4-nitrophenol + D-galactofuranose
-
?
4-nitrophenyl beta-D-galactofuranoside + H2O
-
Streptomyces viridochromogenes 4-nitrophenol + D-galactofuranose
-
?
4-nitrophenyl beta-D-galactofuranoside + H2O
-
Streptomyces chartreusis JHA19 4-nitrophenol + D-galactofuranose
-
?
4-nitrophenyl beta-D-galactofuranoside + H2O
-
Streptomyces sp. JHA19 NRRL F-5140 4-nitrophenol + D-galactofuranose
-
?
4-nitrophenyl beta-D-galactofuranoside + H2O
-
Streptomyces viridochromogenes JHA19 4-nitrophenol + D-galactofuranose
-
?
4-nitrophenyl beta-D-galactofuranoside + H2O
-
Streptomyces sp. NRRL F-5140 4-nitrophenol + D-galactofuranose
-
?
galactomannan + H2O release of galactose residue, galactomannan prepared from Aspergillus fumigatus Streptomyces sp. ?
-
?
galactomannan + H2O release of galactose residue, galactomannan prepared from Aspergillus fumigatus Streptomyces chartreusis ?
-
?
galactomannan + H2O release of galactose residue, galactomannan prepared from Aspergillus fumigatus Streptomyces sp. JHA19 ?
-
?
galactomannan + H2O release of galactose residue, galactomannan prepared from Aspergillus fumigatus Streptomyces viridochromogenes ?
-
?
galactomannan + H2O release of galactose residue, galactomannan prepared from Aspergillus fumigatus Streptomyces chartreusis JHA19 ?
-
?
galactomannan + H2O release of galactose residue, galactomannan prepared from Aspergillus fumigatus Streptomyces sp. JHA19 NRRL F-5140 ?
-
?
galactomannan + H2O release of galactose residue, galactomannan prepared from Aspergillus fumigatus Streptomyces viridochromogenes JHA19 ?
-
?
galactomannan + H2O release of galactose residue, galactomannan prepared from Aspergillus fumigatus Streptomyces sp. NRRL F-5140 ?
-
?
additional information four genes in Streptomyces sp. JHA19 encode Galf-ases. One enzyme (beta-D-galactofuranosidase 2) is a Galf-specific hydrolase, that shows no activity with alpha-L-arabinofuranosides, while the other three (beta-D-galactofuranosidases 1, 3, and 4) are active as alpha-L-arabinofuranosidases (Araf-ases, EC 3.2.1.55) and also as beta-D-galactofuranosidases Streptomyces sp. ?
-
?
additional information four genes in Streptomyces sp. JHA19 encode Galf-ases. One enzyme (beta-D-galactofuranosidase 2) is a Galf-specific hydrolase, that shows no activity with alpha-L-arabinofuranosides, while the other three (beta-D-galactofuranosidases 1, 3, and 4) are active as alpha-L-arabinofuranosidases (Araf-ases, EC 3.2.1.55) and also as beta-D-galactofuranosidases Streptomyces chartreusis ?
-
?
additional information four genes in Streptomyces sp. JHA19 encode Galf-ases. One enzyme (beta-D-galactofuranosidase 2) is a Galf-specific hydrolase, that shows no activity with alpha-L-arabinofuranosides, while the other three (beta-D-galactofuranosidases 1, 3, and 4) are active as alpha-L-arabinofuranosidases (Araf-ases, EC 3.2.1.55) and also as beta-D-galactofuranosidases Streptomyces viridochromogenes ?
-
?
additional information four genes in Streptomyces sp. JHA19 encode Galf-ases. The beta-D-galactofuranosidase 2 enzyme is a Galf-specific hydrolase, that shows no activity with alpha-L-arabinofuranosides, while the other three are also active as alpha-L-arabinofuranosidases (Araf-ases, EC 3.2.1.55) Streptomyces sp. JHA19 ?
-
?
additional information four genes in Streptomyces sp. JHA19 encode Galf-ases. One enzyme (beta-D-galactofuranosidase 2) is a Galf-specific hydrolase, that shows no activity with alpha-L-arabinofuranosides, while the other three (beta-D-galactofuranosidases 1, 3, and 4) are active as alpha-L-arabinofuranosidases (Araf-ases, EC 3.2.1.55) and also as beta-D-galactofuranosidases Streptomyces chartreusis JHA19 ?
-
?
additional information four genes in Streptomyces sp. JHA19 encode Galf-ases. The beta-D-galactofuranosidase 2 enzyme is a Galf-specific hydrolase, that shows no activity with alpha-L-arabinofuranosides, while the other three are also active as alpha-L-arabinofuranosidases (Araf-ases, EC 3.2.1.55) Streptomyces sp. JHA19 NRRL F-5140 ?
-
?
additional information four genes in Streptomyces sp. JHA19 encode Galf-ases. One enzyme (beta-D-galactofuranosidase 2) is a Galf-specific hydrolase, that shows no activity with alpha-L-arabinofuranosides, while the other three (beta-D-galactofuranosidases 1, 3, and 4) are active as alpha-L-arabinofuranosidases (Araf-ases, EC 3.2.1.55) and also as beta-D-galactofuranosidases Streptomyces viridochromogenes JHA19 ?
-
?
additional information four genes in Streptomyces sp. JHA19 encode Galf-ases. One enzyme (beta-D-galactofuranosidase 2) is a Galf-specific hydrolase, that shows no activity with alpha-L-arabinofuranosides, while the other three (beta-D-galactofuranosidases 1, 3, and 4) are active as alpha-L-arabinofuranosidases (Araf-ases, EC 3.2.1.55) and also as beta-D-galactofuranosidases Streptomyces sp. NRRL F-5140 ?
-
?

Synonyms

Synonyms Comment Organism
Araf-ase
-
Streptomyces sp.
Araf-ase
-
Streptomyces chartreusis
Araf-ase
-
Streptomyces viridochromogenes
beta-D-galactofuranosidase
-
Streptomyces sp. JHA19
beta-D-galactofuranosidase 1 UniProt Streptomyces viridochromogenes
beta-D-galactofuranosidase 2
-
Streptomyces sp. JHA19
beta-D-galactofuranosidase 3 UniProt Streptomyces chartreusis
beta-D-galactofuranosidase 4 UniProt Streptomyces sp.
galactofuranose-specific beta-D-galactofuranosidase
-
Streptomyces sp. JHA19
Galf-ase
-
Streptomyces sp.
Galf-ase
-
Streptomyces chartreusis
Galf-ase
-
Streptomyces sp. JHA19
Galf-ase
-
Streptomyces viridochromogenes
Galf-specific Galf-ase
-
Streptomyces sp. JHA19
Galf-specific hydrolase
-
Streptomyces sp. JHA19
More see also EC 3.2.1.55 Streptomyces sp.
More see also EC 3.2.1.55 Streptomyces chartreusis
More see also EC 3.2.1.55 Streptomyces viridochromogenes
ORF0232 locus name Streptomyces viridochromogenes
ORF1110 locus name Streptomyces sp. JHA19
ORF2125 locus name Streptomyces chartreusis
ORF2812 locus name Streptomyces sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Streptomyces sp.
37
-
assay at Streptomyces chartreusis
37
-
assay at Streptomyces sp. JHA19
37
-
assay at Streptomyces viridochromogenes

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
purified recombinant detagged enzyme, pH 5.5, 10 min, stable up to Streptomyces sp. JHA19

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5
-
recombinant enzyme Streptomyces sp. JHA19
5.5
-
assay at Streptomyces sp.
5.5
-
assay at Streptomyces chartreusis
5.5
-
assay at Streptomyces viridochromogenes

General Information

General Information Comment Organism
evolution four genes in Streptomyces sp. JHA19 encode beta-D-galactofuranosidase (Galf-ases). One enzyme (beta-D-galactofuranosidase 2) is a Galf-specific hydrolase, that shows no activity with alpha-L-arabinofuranosides, while the other three (beta-D-galactofuranosidases 1, 3, and 4) are also active as alpha-L-arabinofuranosidases (Araf-ases, EC 3.2.1.55) Streptomyces sp.
evolution four genes in Streptomyces sp. JHA19 encode Galf-ases. One enzyme (beta-D-galactofuranosidase 2) is a Galf-specific hydrolase, that shows no activity with alpha-L-arabinofuranosides, while the other three (beta-D-galactofuranosidases 1, 3, and 4) are also active as alpha-L-arabinofuranosidases (Araf-ases, EC 3.2.1.55) Streptomyces chartreusis
evolution four genes in Streptomyces sp. JHA19 encode Galf-ases. One enzyme (beta-D-galactofuranosidase 2) is a Galf-specific hydrolase, that shows no activity with alpha-L-arabinofuranosides, while the other three (beta-D-galactofuranosidases 1, 3, and 4) are also active as alpha-L-arabinofuranosidases (Araf-ases, EC 3.2.1.55) Streptomyces viridochromogenes