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Literature summary for 3.2.1.145 extracted from
- Purification of an exo-beta-(1->3)-D-galactanase of Irpex lacteus (Polyporus tulipiferae) and its action on arabinogalactan-proteins (1990), J. Biol. Chem., 265, 7207-7215.
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 19.9 | - |
beta-(1-3)-D-galactotriose | - |
Irpex lacteus |  |
| 31.6 | - |
beta-(1-3)-D-galactobiose | - |
Irpex lacteus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 42000 | - |
gel filtration | Irpex lacteus |
| 51000 | - |
native PAGE | Irpex lacteus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| beta-(1-3)-D-galactan + H2O | Irpex lacteus | - |
beta-(1-3)-D-galactan + D-galactose | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Irpex lacteus | - |
Polyporus tulipiferae | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| DEAE-cellulose, CM-Toyopearl, hydroylapatite, electrofocusing, purified from commercial driselase | Irpex lacteus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 87.8 | - |
- |
Irpex lacteus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| beta-(1-3)-D-galactan + H2O | - |
Irpex lacteus | beta-(1-3)-D-galactan + D-galactose | - |
? | |
| beta-(1-3)-D-galactotriose + H2O | - |
Irpex lacteus | beta-(1-3)-D-galactobiose + D-galactose | - |
? | |
| beta-(1-3)-galactobiose + H2O | - |
Irpex lacteus | D-galactose | - |
? | |
| beta-D-Gal-[(1->3)-beta-D-Gal]n + H2O | only beta-(1, 3) oligomers act as substrates | Irpex lacteus | beta-D-Gal-[(1->3)-beta-D-Gal]n-1 + D-galactose | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 51000, SDS-PAGE | Irpex lacteus |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | 48 | retains full activity up to 48°C, completely inactivated at 60°C | Irpex lacteus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | 48 | retains full activity up to 48°C, completely inactivated at 60°C | Irpex lacteus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4.6 | - |
enzyme action on beta-(1-3)-D-galactan | Irpex lacteus |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 3.5 | 7.5 | after incubation for 24 h at 4°C | Irpex lacteus |
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