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Literature summary for 3.2.1.143 extracted from

  • Araiza-Cervantes, C.; Meza-Carmen, V.; Martinez-Cadena, G.; Roncero, M.; Reyna-Lopez, G.; Franco, B.
    Biochemical and genetic analysis of a unique poly(ADP-ribosyl) glycohydrolase (PARG) of the pathogenic fungus Fusarium oxysporum f. sp. lycopersici (2018), Antonie Van Leeuwenhoek, 111, 285-295 .
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Cloned(Commentary)

Cloned (Comment) Organism
cloning from genomic DNA, DNA and amino acid sequence determination and analysis, recombinant expression of His6-tagged enzyme in Escherichia coli strain BL21(DE3), quantitative RT-PCR expression analysis Fusarium oxysporum f. sp. lycopersici

Protein Variants

Protein Variants Comment Organism
additional information construction of a DELTAparg deletion mutant. The mutant DELTAparg strain, in the presence of H2O2, exhibits a reduced randomly amplified polymorphic DNA (RAPD) pattern, suggesting that DNA is damaged in this strain and repair is impaired Fusarium oxysporum f. sp. lycopersici

Organism

Organism UniProt Comment Textmining
Fusarium oxysporum f. sp. lycopersici A0A0D2XPQ9
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Fusarium oxysporum f. sp. lycopersici 4287 A0A0D2XPQ9
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Fusarium oxysporum f. sp. lycopersici CBS 123668 A0A0D2XPQ9
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Fusarium oxysporum f. sp. lycopersici FGSC 9935 A0A0D2XPQ9
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Fusarium oxysporum f. sp. lycopersici NRRL 34936 A0A0D2XPQ9
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Purification (Commentary)

Purification (Comment) Organism
recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography Fusarium oxysporum f. sp. lycopersici

Source Tissue

Source Tissue Comment Organism Textmining
additional information tomato seedlings of non-resistant Money-maker cultivar plants are inoculated with wild-type and DELTAparg mutant Fusarium oxysporum f. sp. lycopersici
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mycelium
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 Fusarium oxysporum f. sp. lycopersici
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Synonyms

Synonyms Comment Organism
FOXG_05947
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 Fusarium oxysporum f. sp. lycopersici
PARG
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 Fusarium oxysporum f. sp. lycopersici
poly(ADP-ribosyl) glycohydrolase
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 Fusarium oxysporum f. sp. lycopersici

General Information

General Information Comment Organism
malfunction the wild-type strain with or without H2O2 shows no evident changes in the randomly amplified polymorphic DNA, RAPD, pattern. The mutant DELTAparg strain, in the presence of H2O2, exhibits a reduced pattern, suggesting that DNA is damaged in this strain and repair is impaired. No morphological differences in color, growth rate or morphology are observed for the mutant strain on solid medium as compared with the wild-type strain Fusarium oxysporum f. sp. lycopersici
physiological function degradation of poly(ADP-ribose), PAR, is catalyzed by poly(ADP-ribose) glycohydrolase (PARG) by endo- and exoglycosidase reactions that release products of variable length and ADPribose monomers. The poly(ADP-ribosyl) glycohydrolase, PARG, protein of Fusarium oxysporum f. sp. lycopersici is involved in DNA repair and does not act in pathogenicity as an effector. The organism encodes for only one PARG enzyme, and this is responsible for the total cellular PARG activity Fusarium oxysporum f. sp. lycopersici