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Literature summary for 3.2.1.143 extracted from

  • Stowell, A.I.; James, D.I.; Waddell, I.D.; Bennett, N.; Truman, C.; Hardern, I.M.; Ogilvie, D.J.
    A high-throughput screening-compatible homogeneous time-resolved fluorescence assay measuring the glycohydrolase activity of human poly(ADP-ribose) glycohydrolase (2016), Anal. Biochem., 503, 58-64 .
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
gene PARG, single gene, recombinant expression of C-terminally His-tagged hPARG in Escherichia coli BL21(DE3) Gold Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
additional information development of a high-throughput homogeneous time-resolved fluorescence (HTRF) assay method allows high-throughput screening for the identification and advancement of multiple validated series of tool compounds for PARG inhibition Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
poly(ADP-ribose) + H2O Homo sapiens
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?
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?

Organism

Organism UniProt Comment Textmining
Homo sapiens Q86W56
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-

Purification (Commentary)

Purification (Comment) Organism
recombinant C-terminally His-tagged hPARG from Escherichia coli BL21(DE3) Gold by nickel affinity chromatography and gel filtration Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information development of a high-throughput homogeneous time-resolved fluorescence (HTRF) assay method, 6His-TEV-PAR(32:1)PARP-1(2-1014) substrate, combinantion with the activity of poly(ADP-ribose) polymerase, PARP. Enzyme PARG binds to PARylated PARP-1 and cleaves off the PAR moiety, which activates PARP-1 preventing the production of a fluorescent signal. Inactive PARP-1 leads to the production of the measurable fluoresecnt signal, mechanism and evaluation, overview Homo sapiens ?
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?
poly(ADP-ribose) + H2O
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Homo sapiens ?
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?

Synonyms

Synonyms Comment Organism
PARG
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Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
26
-
assay at Homo sapiens

General Information

General Information Comment Organism
physiological function poly(ADP-ribosyl)ation (PARylation) is a transient posttranslational modification that generates a signaling mechanism with diverse roles within molecular and cellular processes. PAR chains remaining from DNA repair are broken down by the enzyme poly(ADP-ribose) glycohydrolase (PARG). PARG catalyzes the hydrolysis of endo- and exoglycosidic bonds within the poly(ADP-ribose) (PAR) polymers Homo sapiens