Literature summary for 3.2.1.143 extracted from

Slade, D.; Dunstan, M.; Barkauskaite, E.; Weston, R.; Lafite, P.; Dixon, N.; Ahel, M.; Leys, D.; Ahel, I.
The structure and catalytic mechanism of a poly(ADP-ribose) glycohydrolase (2011), Nature, 477, 616-622.

Search for more literature for 3.2.1.143

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli	Homo sapiens
expressed in Escherichia coli	Thermomonospora curvata

Crystallization (Commentary)

Crystallization (Comment)	Organism
in complexes with ADP-ribose and inhibitor adenosine 5'-diphosphate (hydroxymethyl) pyrrolidinediol	Thermomonospora curvata

Protein Variants

Protein Variants	Comment	Organism
A874W	the mutant shows about 45% activity compared to the wild type enzyme	Homo sapiens
E114A	the mutant shows about 2% activity compared to the wild type enzyme	Thermomonospora curvata
E115A	inactive	Thermomonospora curvata
E755A	inactive	Homo sapiens
E756A	inactive	Homo sapiens
F227A	inactive	Thermomonospora curvata
F875A	the mutant shows about 1% activity compared to the wild type enzyme	Homo sapiens
N740A	the mutant shows about 30% activity compared to the wild type enzyme	Homo sapiens
S298K	the mutant shows about 2% activity compared to the wild type enzyme	Thermomonospora curvata
V226W	the mutant shows about 5% activity compared to the wild type enzyme	Thermomonospora curvata

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	-	-
Thermomonospora curvata	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
poly(ADP-ribose) + H2O	-	Homo sapiens	ADP-ribose oligomer + ADP-ribose	-	?
poly(ADP-ribose) + H2O	-	Thermomonospora curvata	ADP-ribose oligomer + ADP-ribose	-	?

Synonyms

Synonyms	Comment	Organism
PARG	-	Homo sapiens
PARG	-	Thermomonospora curvata

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Release 2023.1 (January 2023)