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Literature summary for 3.2.1.143 extracted from

  • Botta, D.; Jacobson, M.K.
    Identification of a regulatory segment of poly(ADP-ribose) glycohydrolase (2010), Biochemistry, 49, 7674-7682.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli K12 TB1 and Rosetta ells Homo sapiens

Protein Variants

Protein Variants Comment Organism
L11D the mutation increases enzyme activity to 148% Homo sapiens
L11D/L13D the mutant almost entirely abolishes enzyme activity (4% activity) Homo sapiens
L11D/L13D/L14D the mutant results in no detectable activity (less than 0.1% activity) Homo sapiens
additional information deletion of the regulatory segment/MTS from full-length human PARG111 results in a complete loss of activity Homo sapiens
R10A the mutation results in a significant increase in activity (144%) Homo sapiens
R2A/R3A/R6A/R10A the mutant shows 113% enzyme activity Homo sapiens
R3A the mutant shows 105% enzyme activity Homo sapiens
R6A the mutation results in a significant increase in activity (248%) Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
nucleus full-length enzyme PARG111 Homo sapiens 5634
-

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography Homo sapiens

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.00102
-
full length enzyme PARG111, pH and temperature not specified in the publication Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
poly(ADP-ribose) + H2O
-
Homo sapiens ADP-ribose oligomer + ADP-ribose
-
?

Synonyms

Synonyms Comment Organism
PARG
-
Homo sapiens
PARG111 full-length enzyme Homo sapiens

General Information

General Information Comment Organism
physiological function coordinate regulation of PARP-1 and -2 and PARG is required for cellular responses to genotoxic stress Homo sapiens