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Literature summary for 3.2.1.143 extracted from

  • Erdelyi, K.; Bai, P.; Kovacs, I.; Szabo, E.; Mocsar, G.; Kakuk, A.; Szabo, C.; Gergely, P.; Virag, L.
    Dual role of poly(ADP-ribose) glycohydrolase in the regulation of cell death in oxidatively stressed A549 cells (2009), FASEB J., 23, 3553-3563.
    View publication on PubMedView publication on EuropePMC

Organism

Organism UniProt Comment Textmining
Homo sapiens
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Source Tissue

Source Tissue Comment Organism Textmining
A-549 cell
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Homo sapiens
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General Information

General Information Comment Organism
physiological function generation of A549 lung adenocarcinoma cell lines with stably suppressed PARG and poly(ADP-ribose) polymerase PARP-1 expression, i.e. shPARG and shPARP1 cell lines, respectively. shPARG cells accumulate large amounts of poly-(ADP-ribosyl)ated proteins and exhibit reduced PARP activation. Hydrogen peroxide-induced cell death is regulated by PARG in a dual fashion. Whereas the shPARG cell line is resistant to the necrotic effect of high concentrations of hydrogen peroxide, these cells exhibit stronger apoptotic response. Both shPARP1 and especially shPARG cells display a delayed repair of DNA breaks and exhibit reduced clonogenic survival following hydrogen peroxide treatment. Translocation of apoptosis-inducing factor cannot be observed, but cells can be saved by methyl pyruvate and alpha-ketoglutarate Homo sapiens