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Literature summary for 3.2.1.143 extracted from
- Purification and characterization of poly(ADP-ribose) glycohydrolase. Different modes of action on large and small poly(ADP-ribose) (1986), J. Biol. Chem., 261, 14902-14911.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| adenosine 5'-tetraphosphate | 5 mM, slight activation | Bos taurus |  |
| ADP | 5 mM, slight activation | Bos taurus | |
| ADP-glucose | 5 mM, slight activation | Bos taurus | |
| AMP | 5 mM, slight activation | Bos taurus | |
| ATP | 5 mM, slight activation | Bos taurus | |
| NADH | 5 mM, slight activation | Bos taurus | |
| NADP+ | 5 mM, slight activation | Bos taurus | |
| NADPH | 5 mM, slight activation | Bos taurus | |
| P1,P5-di(adenosine-5')pentaphosphate | 5 mM, slight activation | Bos taurus | |
General Stability
| General Stability | Organism |
|---|---|
| poly(ADP-ribose) protects the enzyme from thermal inactivation | Bos taurus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2':3'-cyclic NADP+ | - |
Bos taurus | |
| acetone | 20%, 95% inhibition | Bos taurus | |
| adenosine 3':5'-cyclic monophosphate | - |
Bos taurus | |
| ADP-ribose | 5 mM, 55% inhibition | Bos taurus | |
| DNA | denatured; no inhibition by native DNA | Bos taurus | |
| ethanol | 20%, 91% inhibition | Bos taurus | |
| histone | histone H1, H2A, H2B, H3 and H4 | Bos taurus | |
| Poly(A) | - |
Bos taurus | |
| RNA | calf liver RNA | Bos taurus | |
| SDS | 0.01%, 96% inhibition | Bos taurus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0003 | - |
Poly(ADP-ribose) | - |
Bos taurus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| nucleus | - |
Bos taurus | 5634 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| KCl | 100 mM, slight activation | Bos taurus | |
| NaCl | 100 mM, slight activation | Bos taurus | |
| Sodium phosphate | 100 mM, slight activation | Bos taurus | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| poly(ADP-ribose)n + H2O | Bos taurus | regulates differentially the levels of large and small poly(ADP-ribose) in the cell | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bos taurus | - |
calf | - |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| thymus | - |
Bos taurus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 85 | - |
- |
Bos taurus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, retains 90-100% of its activity for at least 1.5 years | Bos taurus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| poly(ADP-ribose)n + H2O | n greater 20, degradation proceeds in a biphasic as well as bimodal manner | Bos taurus | ADP-ribose | - |
? | |
| poly(ADP-ribose)n + H2O | regulates differentially the levels of large and small poly(ADP-ribose) in the cell | Bos taurus | ? | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
- |
Bos taurus |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | 50 | 30°C: about 25% of maximal activity, 55°C: about 50% of maximal activity, no activity at 60°C | Bos taurus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| additional information | - |
poly(ADP-ribose) protects the enzyme from thermal inactivation | Bos taurus |
| 30 | - |
10 min, stable | Bos taurus |
| 45 | - |
10 min, about 10% loss of activity | Bos taurus |
| 50 | - |
10 min, complete inactivation | Bos taurus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 90 | - |
Poly(ADP-ribose) | - |
Bos taurus | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | 7.5 | - |
Bos taurus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 8 | about 45% of maximal activity at pH 6.0 and 8.0 | Bos taurus |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 9 | 37°C, 10 min, stable | Bos taurus |
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