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Literature summary for 3.2.1.143 extracted from

  • Miwa, M.; Tanaka, M.; Matsushima, T.; Sugimura, T.
    Purification and properties of a glycohydrolase from calf thymus splitting ribose-ribose linkages of poly(adenosine diphosphate ribose) (1974), J. Biol. Chem., 249, 3475-3482.
    View publication on PubMed
Search for more literature for 3.2.1.143

Activating Compound

Activating Compound Comment Organism Structure
2-mercaptoethanol 10 mM, required for maximal activity Bos taurus
dithiothreitol 10 mM, required for maximal activity Bos taurus

Inhibitors

Inhibitors Comment Organism Structure
adenosine 3':5'-cyclic monophosphate 0.3 mM, 50% inhibition Bos taurus
adenosine cyclic 2':3'-monophosphate 10 mM, 23% inhibition Bos taurus
ADP 10 mM, 39% inhibition Bos taurus
ADP-ribose 1.1 mM, 50% inhibition Bos taurus
AMP 10 mM, 64% inhibition Bos taurus
ATP 10 mM, 23% inhibition Bos taurus
guanosine cyclic 3':5'-monophosphate 10 mM, 42% inhibition Bos taurus
HgCl2
-
 Bos taurus
histone histone f2a, f2b and f3. Inhibition by histone f2a is reversed by DNA Bos taurus
N6,O2'-dibutyryl adenosine cyclic 3':5'-monophosphate 10 mM, 11% inhibition Bos taurus
N6-monobutyryl adenosine cyclic 3':5'-monophosphate 10 mM, 50% inhibition Bos taurus
NAD+ 10 mM, 40% inhibition Bos taurus
p-chloromercuribenzenesulfonate 0.01 mM Bos taurus
poly(L-Lys)
-
 Bos taurus
protamine
-
 Bos taurus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00058
-
 (ADP-ribose)n
-
 Bos taurus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
48000
-
 gel filtration Bos taurus
53000
-
 sucrose density gradient centrifugation Bos taurus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
poly(ADP-ribose)n + H2O Bos taurus the enzyme may regulate functionally the chain length of poly(ADP-ribose) which plays a role in DNA synthesis or in the structure of chromatin ?
-
 ?

Organism

Organism UniProt Comment Textmining
Bos taurus
-
 calf
-

Purification (Commentary)

Purification (Comment) Organism
-
 Bos taurus

Source Tissue

Source Tissue Comment Organism Textmining
thymus
-
 Bos taurus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.182
-
-
 Bos taurus

Storage Stability

Storage Stability Organism
-80°C, stable for 3 months Bos taurus
4°C, 20% loss of activity after 1 month, addition of 0.1 mg/ml bovine serum albumin or 0.1 M NaCl to the diluted enzyme solution stabilizes the enzyme activity Bos taurus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
poly(ADP-ribose)n + H2O
-
 Bos taurus ADP-ribose
-
 ?
poly(ADP-ribose)n + H2O the enzyme may regulate functionally the chain length of poly(ADP-ribose) which plays a role in DNA synthesis or in the structure of chromatin Bos taurus ?
-
 ?

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 activity is less in Tris-HCl buffer than in sodium phosphate buffer or N-2-hydroxyethylpiperazine-N-ethanesulfonic acid-NaOH buffer Bos taurus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.3
-
 adenosine 3':5'-cyclic monophosphate
-
 Bos taurus
1.1
-
 ADP-ribose
-
 Bos taurus
10
-
 N6-monobutyryl adenosine cyclic 3':5'-monophosphate
-
 Bos taurus