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Literature summary for 3.2.1.143 extracted from
- Characterization of two forms of poly(ADP-ribose) glycohydrolase in guniea pig liver (1991), Biochemistry, 30, 5907-5912.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| ADP-ribose | inhibits poly(ADP-ribose) glycohydrolase II more strongly than poly(ADP-ribose) glycohydrolase I | Cavia porcellus |  |
| cAMP | inhibits poly(ADP-ribose) glycohydrolase II more strongly than poly(ADP-ribose) glycohydrolase I | Cavia porcellus | |
| KCl | 100 mM, 50% inhibition, poly(ADP-ribose) glycohydrolase II | Cavia porcellus | |
| NaCl | 50-100 mM, poly(ADP-ribose) glycohydrolase II | Cavia porcellus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00058 | - |
(ADP-ribose)n | average chain length n = 27, poly(ADP-ribose) glycohydrolase I | Cavia porcellus | |
| 0.0011 | - |
(ADP-ribose)n | average chain length n = 21, poly(ADP-ribose) glycohydrolase I | Cavia porcellus | |
| 0.002 | - |
(ADP-ribose)n | average chain length n = 15, poly(ADP-ribose) glycohydrolase I | Cavia porcellus | |
| 0.0031 | - |
(ADP-ribose)n | average chain length n = 27, poly(ADP-ribose) glycohydrolase II | Cavia porcellus | |
| 0.004 | - |
(ADP-ribose)n | average chain length n = 21, poly(ADP-ribose) glycohydrolase II | Cavia porcellus | |
| 0.0045 | - |
(ADP-ribose)n | average chain length n = 6.7, poly(ADP-ribose) glycohydrolase I | Cavia porcellus | |
| 0.0048 | - |
(ADP-ribose)n | average chain length n = 15, poly(ADP-ribose) glycohydrolase II | Cavia porcellus | |
| 0.0066 | - |
(ADP-ribose)n | average chain length n = 6.7, poly(ADP-ribose) glycohydrolase II | Cavia porcellus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytoplasm | poly(ADP-ribose) glycohydrolase II | Cavia porcellus | 5737 | - |
| nucleus | poly(ADP-ribose) glycohydrolase I | Cavia porcellus | 5634 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| KCl | 50-100 mM, stimulates poly(ADP-ribose) glycohydrolase I | Cavia porcellus | |
| NaCl | 50-100 mM, stimulates poly(ADP-ribose) glycohydrolase I | Cavia porcellus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 57000 | - |
poly(ADP-ribose) glycohydrolase II, gel filtration | Cavia porcellus |
| 59500 | - |
1 * 59500, poly(ADP-ribose) glycohydrolase II, SDS-PAGE | Cavia porcellus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Cavia porcellus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| cytoplasmic enzyme | Cavia porcellus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | - |
Cavia porcellus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 13.4 | - |
poly(ADP-ribose) glycohydrolase II | Cavia porcellus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, stable for at least 6 months | Cavia porcellus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| poly(ADP-ribose)n + H2O | - |
Cavia porcellus | ADP-ribose | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 59500, poly(ADP-ribose) glycohydrolase II, SDS-PAGE | Cavia porcellus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
poly(ADP-ribose) glycohydrolase I | Cavia porcellus |
| 7.4 | - |
poly(ADP-ribose) glycohydrolase II | Cavia porcellus |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 9 | optimal stability | Cavia porcellus |
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