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Literature summary for 3.2.1.142 extracted from

  • Qiao, Y.; Peng, Q.; Yan, J.; Wang, H.; Ding, H.; Shi, B.
    Gene cloning and enzymatic characterization of alkali-tolerant type I pullulanase from Exiguobacterium acetylicum (2015), Lett. Appl. Microbiol., 60, 52-59.
    View publication on PubMed
Search for more literature for 3.2.1.142

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Exiguobacterium acetylicum

Inhibitors

Inhibitors Comment Organism Structure
2-mercaptoethanol 5 mM, 51% residual activity Exiguobacterium acetylicum
Cu2+ 5 mM, 67% residual activity Exiguobacterium acetylicum
EDTA 5 mM, 72% residual activity Exiguobacterium acetylicum
Mg2+ 5 mM, 90% residual activity Exiguobacterium acetylicum
SDS 5 mM, 46% residual activity Exiguobacterium acetylicum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 pullulan Km value 0.12 mg/ml, pH 6.0, 50°C Exiguobacterium acetylicum
additional information
-
 soluble starch Km value 0.69 mg/ml,pH 6.0, 50°C Exiguobacterium acetylicum

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ 5 mM, 198% of initial activity Exiguobacterium acetylicum
Fe2+ 5 mM, 121% of initial activity Exiguobacterium acetylicum
Mn2+ 5 mM, 136% of initial activity Exiguobacterium acetylicum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
95800
-
-
 Exiguobacterium acetylicum
100000
-
 PAGE Exiguobacterium acetylicum

Organism

Organism UniProt Comment Textmining
Exiguobacterium acetylicum I3WU34
-
-
Exiguobacterium acetylicum YH5 I3WU34
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
amylopectin + H2O 6.0% of the activity with pullulan Exiguobacterium acetylicum maltotriose + maltose + maltooligosaccharides
-
 ?
beta-limited dextrin + H2O 19.7% of the activity with pullulan Exiguobacterium acetylicum maltotriose + maltose + maltooligosaccharides
-
 ?
glycogen + H2O 6.1% of the activity with pullulan Exiguobacterium acetylicum maltotriose + maltose + maltooligosaccharides
-
 ?
additional information no substrates: maltotriose, maltose. the enzyme is strictly restricted to hydrolysis of 1->6-linked glucosidic linkages. It also acts as type I pullulanase, EC 3.2.1.41 Exiguobacterium acetylicum ?
-
 ?
pullulan + H2O
-
 Exiguobacterium acetylicum maltotriose + ? complete conversion to maltotriose ?
soluble starch + H2O 12.8% of the activity with pullulan Exiguobacterium acetylicum maltotriose + maltose + maltooligosaccharides
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 100000, SDS-PAGE, 1 * 95800, calculated Exiguobacterium acetylicum

Synonyms

Synonyms Comment Organism
Pul3YH5
-
 Exiguobacterium acetylicum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
 Exiguobacterium acetylicum

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 pH 5-8, 10 min, more than 70% of maximum activity Exiguobacterium acetylicum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
 Exiguobacterium acetylicum

pH Stability

pH Stability pH Stability Maximum Comment Organism
4 10 30 min, more than 93% residual activity Exiguobacterium acetylicum

pI Value

Organism Comment pI Value Maximum pI Value
Exiguobacterium acetylicum calculated
-
 6.5