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Literature summary for 3.2.1.142 extracted from

  • Mangan, D.; McCleary, B.; Cornaggia, C.; Ivory, R.; Rooney, E.; McKie, V.
    Colourimetric and fluorimetric substrates for the assay of limit dextrinase (2015), J. Cereal Sci., 62, 50-57.
No PubMed abstract available
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Application

Application Comment Organism
analysis substrate 4,6-O-benzylidene-2-chloro-4-nitrophenyl-beta-63-alpha-D-maltotriosyl-maltotrioside (BzCNPG3G3, Hexachrom) is not susceptible to transglycosylation and serves amiably as a routine quantitative assay tool with the potential to run kinetic assays due to the low pKa (about 5.5) of the chromogenic moiety. Substrate 4,6-O-benzylidene-4-methylumbelliferyl-beta-63-alpha-D-maltotriosyl-maltotrioside (BzMUG3G3, Hexafluor) is susceptible to transglycosylation. Both substrates are applicable to industrial standard assays Oryza sativa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.052
-
 4,6-O-benzylidene-4-methylumbelliferyl-beta-63-alpha-D-maltotriosyl-maltotrioside pH.0, 40°C Oryza sativa
0.192
-
 4,6-O-benzylidene-2-chloro-4-nitrophenyl-beta-63-alpha-D-maltotriosyl-maltotrioside pH.0, 40°C Oryza sativa

Organism

Organism UniProt Comment Textmining
Oryza sativa
-
-
-

Purification (Commentary)

Purification (Comment) Organism
from rice grain Oryza sativa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4,6-O-benzylidene-2-chloro-4-nitrophenyl-beta-63-alpha-D-maltotriosyl-maltotrioside + H2O substrate is not susceptible to transglycosylation and may serve as a routine quantitative assay tool Oryza sativa 4,6-O-benzylidene-D-maltotriose + 4-nitrophenyl beta-D-maltotriose
-
 ?
4,6-O-benzylidene-4-methylumbelliferyl-beta-63-alpha-D-maltotriosyl-maltotrioside substrate is susceptible to transglycosylation and may serve as a routine quantitative assay tool Oryza sativa 4,6-O-benzylidene-D-maltotriose + 4-methylumbelliferyl beta-D-maltotriose
-
 ?
additional information the enzyme displays transglycosylation activity Oryza sativa ?
-
 ?