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Literature summary for 3.2.1.142 extracted from
- Purification and characterization of limit dextrinase from Pisum sativum L. (1980), Carbohydr. Res., 83, 109-118.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| cyclohexaamylose | competitive inhibition | Pisum sativum |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 3.24 | - |
amylopectin beta-limit dextrin | - |
Pisum sativum |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 180000 | - |
gel filtration | Pisum sativum |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| polysaccharide + H2O | Pisum sativum | natural substrates appear to be alpha-limit dextrins | oligosaccharides | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pisum sativum | - |
ungerminated peas | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| acetone precipitation, affinity chromatography | Pisum sativum |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 1.08 | - |
- |
Pisum sativum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| amylopectin beta-limit dextrin + H2O | - |
Pisum sativum | maltotriose + maltose + maltooligosaccharides | - |
? | |
| polysaccharide + H2O | oligosaccharides containing maltosyl or larger side chains are hydrolysed as well as amylopectin and amylopectin beta-limit dextrin, oligo- and poly-saccharides containing single D-glucosyl groups attached by an alpha-(1,6) linkage to the rest of the molecule are not hydrolysed, glycogen is not a substrate | Pisum sativum | oligosaccharides | - |
? | |
| polysaccharide + H2O | natural substrates appear to be alpha-limit dextrins | Pisum sativum | oligosaccharides | - |
? | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.8 | 6.2 | with both pullulan and amylopectin beta-limit dextrin as substrates | Pisum sativum |
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