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Literature summary for 3.2.1.141 extracted from
- Interaction between trehalose and MTHase from Sulfolobus solfataricus studied by theoretical computation and site-directed mutagenesis (2013), PLoS One, 8, e68565.
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| D156A | kcat/Km for maltooligosyltrehalose is 3.8fold lower than wild-type value | Saccharolobus solfataricus |
| E450A | kcat/Km for maltooligosyltrehalose is 1.2fold lower than wild-type value | Saccharolobus solfataricus |
| H195A | kcat/Km for maltooligosyltrehalose is 357fold lower than wild-type value | Saccharolobus solfataricus |
| R447A | kcat/Km for maltooligosyltrehalose is 6.5fold lower than wild-type value | Saccharolobus solfataricus |
| Y155A | kcat/Km for maltooligosyltrehalose is 160fold lower than wild-type value | Saccharolobus solfataricus |
| Y155F | kcat/Km for maltooligosyltrehalose is 33fold lower than wild-type value | Saccharolobus solfataricus |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 4.18 | - |
maltooligosyltrehalose | pH 5, 60°C, calculated as the amount of trehalose released from the substrate, mutant enzyme H195A | Saccharolobus solfataricus |  |
| 5.02 | - |
maltooligosyltrehalose | pH 5, 60°C, calculated as the amount of trehalose released from the substrate, mutant enzyme Y155F | Saccharolobus solfataricus | |
| 5.22 | - |
maltooligosyltrehalose | pH 5, 60°C, calculated as the amount of trehalose released from the substrate, wild-type enzyme | Saccharolobus solfataricus | |
| 7.48 | - |
maltooligosyltrehalose | pH 5, 60°C, calculated as the amount of trehalose released from the substrate, mutant enzyme E450A | Saccharolobus solfataricus | |
| 10.7 | - |
maltooligosyltrehalose | pH 5, 60°C, calculated as the amount of trehalose released from the substrate, mutant enzyme R447A | Saccharolobus solfataricus | |
| 12.9 | - |
maltooligosyltrehalose | pH 5, 60°C, calculated as the amount of trehalose released from the substrate, mutant enzyme D156A | Saccharolobus solfataricus | |
| 39.2 | - |
maltooligosyltrehalose | pH 5, 60°C, calculated as the amount of trehalose released from the substrate, mutant enzyme Y155A | Saccharolobus solfataricus | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Saccharolobus solfataricus | - |
- |
- |
| Saccharolobus solfataricus KM1 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Saccharolobus solfataricus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| maltooligosyltrehalose + H2O | - |
Saccharolobus solfataricus | trehalose + ? | - |
? | |
| maltooligosyltrehalose + H2O | - |
Saccharolobus solfataricus KM1 | trehalose + ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| maltooligosyltrehalose trehalohydrolase | - |
Saccharolobus solfataricus |
| MTHase | - |
Saccharolobus solfataricus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
assay at | Saccharolobus solfataricus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 2.15 | - |
maltooligosyltrehalose | pH 5, 60°C, calculated as the amount of trehalose released from the substrate, mutant enzyme H195A | Saccharolobus solfataricus | |
| 27.8 | - |
maltooligosyltrehalose | pH 5, 60°C, calculated as the amount of trehalose released from the substrate, mutant enzyme Y155F | Saccharolobus solfataricus | |
| 44.8 | - |
maltooligosyltrehalose | pH 5, 60°C, calculated as the amount of trehalose released from the substrate, mutant enzyme Y155A | Saccharolobus solfataricus | |
| 303 | - |
maltooligosyltrehalose | pH 5, 60°C, calculated as the amount of trehalose released from the substrate, mutant enzyme R447A | Saccharolobus solfataricus | |
| 608 | - |
maltooligosyltrehalose | pH 5, 60°C, calculated as the amount of trehalose released from the substrate, mutant enzyme D156A | Saccharolobus solfataricus | |
| 947 | - |
maltooligosyltrehalose | pH 5, 60°C, calculated as the amount of trehalose released from the substrate, wild-type enzyme | Saccharolobus solfataricus | |
| 1070 | - |
maltooligosyltrehalose | pH 5, 60°C, calculated as the amount of trehalose released from the substrate, mutant enzyme E450A | Saccharolobus solfataricus | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5 | - |
assay at | Saccharolobus solfataricus |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.51 | - |
maltooligosyltrehalose | pH 5, 60°C, calculated as the amount of trehalose released from the substrate, mutant enzyme H195A | Saccharolobus solfataricus | |
| 1.14 | - |
maltooligosyltrehalose | pH 5, 60°C, calculated as the amount of trehalose released from the substrate, mutant enzyme Y155A | Saccharolobus solfataricus | |
| 5.54 | - |
maltooligosyltrehalose | pH 5, 60°C, calculated as the amount of trehalose released from the substrate, mutant enzyme Y155F | Saccharolobus solfataricus | |
| 28.2 | - |
maltooligosyltrehalose | pH 5, 60°C, calculated as the amount of trehalose released from the substrate, mutant enzyme R447A | Saccharolobus solfataricus | |
| 47.3 | - |
maltooligosyltrehalose | pH 5, 60°C, calculated as the amount of trehalose released from the substrate, mutant enzyme D156A | Saccharolobus solfataricus | |
| 144 | - |
maltooligosyltrehalose | pH 5, 60°C, calculated as the amount of trehalose released from the substrate, mutant enzyme E450A | Saccharolobus solfataricus | |
| 182 | - |
maltooligosyltrehalose | pH 5, 60°C, calculated as the amount of trehalose released from the substrate, wild-type enzyme | Saccharolobus solfataricus | |
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