Inhibitors | Comment | Organism | Structure |
---|---|---|---|
CuSO4 | - |
Saccharolobus solfataricus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
4.7 | - |
maltosylpentaosyltrehalose | pH 5.5, 60°C | Saccharolobus solfataricus | |
5.6 | - |
maltosyltetraosyltrehalose | pH 5.5, 60°C | Saccharolobus solfataricus | |
5.8 | - |
maltosyltriosyltrehalose | pH 5.5, 60°C | Saccharolobus solfataricus | |
22.4 | - |
maltosyltrehalose | pH 5.5, 60°C | Saccharolobus solfataricus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
61000 | - |
x * 61000, SDS-PAGE | Saccharolobus solfataricus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharolobus solfataricus | Q55088 | - |
- |
Saccharolobus solfataricus KM1 | Q55088 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Saccharolobus solfataricus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
maltosylpentaosyltrehalose + H2O | 93.4% of the activity compared to maltosyltetraosyltrehalose, hydrolyzes glycosyltrehaloses from the side of the reducing end only by cleavage of the alpha-1,4 glucosidic bond adjacent to the alpha-1,1 glucosidic bond to liberate trehalose | Saccharolobus solfataricus | trehalose + maltopentaose | - |
? | |
maltosylpentaosyltrehalose + H2O | 93.4% of the activity compared to maltosyltetraosyltrehalose, hydrolyzes glycosyltrehaloses from the side of the reducing end only by cleavage of the alpha-1,4 glucosidic bond adjacent to the alpha-1,1 glucosidic bond to liberate trehalose | Saccharolobus solfataricus KM1 | trehalose + maltopentaose | - |
? | |
maltosyltetraosyltrehalose + H2O | hydrolyzes glycosyltrehaloses from the side of the reducing end only by cleavage of the alpha-1,4 glucosidic bond adjacent to the alpha-1,1 glucosidic bond to liberate trehalose | Saccharolobus solfataricus | trehalose + maltotetraose | - |
? | |
maltosyltetraosyltrehalose + H2O | hydrolyzes glycosyltrehaloses from the side of the reducing end only by cleavage of the alpha-1,4 glucosidic bond adjacent to the alpha-1,1 glucosidic bond to liberate trehalose | Saccharolobus solfataricus KM1 | trehalose + maltotetraose | - |
? | |
maltosyltrehalose + H2O | 14.4% of the activity compared to maltosyltetraosyltrehalose, hydrolyzes glycosyltrehaloses from the side of the reducing end only by cleavage of the alpha-1,4 glucosidic bond adjacent to the alpha-1,1 glucosidic bond to liberate trehalose | Saccharolobus solfataricus | trehalose + maltose | - |
? | |
maltosyltrehalose + H2O | 14.4% of the activity compared to maltosyltetraosyltrehalose, hydrolyzes glycosyltrehaloses from the side of the reducing end only by cleavage of the alpha-1,4 glucosidic bond adjacent to the alpha-1,1 glucosidic bond to liberate trehalose | Saccharolobus solfataricus KM1 | trehalose + maltose | - |
? | |
maltosyltriosyltrehalose + H2O | 91.6% of the activity compared to maltosyltetraosyltrehalose, hydrolyzes glycosyltrehaloses from the side of the reducing end only by cleavage of the alpha-1,4 glucosidic bond adjacent to the alpha-1,1 glucosidic bond to liberate trehalose | Saccharolobus solfataricus | trehalose + maltotriose | - |
? | |
maltosyltriosyltrehalose + H2O | 91.6% of the activity compared to maltosyltetraosyltrehalose, hydrolyzes glycosyltrehaloses from the side of the reducing end only by cleavage of the alpha-1,4 glucosidic bond adjacent to the alpha-1,1 glucosidic bond to liberate trehalose | Saccharolobus solfataricus KM1 | trehalose + maltotriose | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 61000, SDS-PAGE | Saccharolobus solfataricus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
assay at | Saccharolobus solfataricus |
70 | 85 | - |
Saccharolobus solfataricus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
85 | - |
6 h, stable | Saccharolobus solfataricus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4.5 | 5.5 | - |
Saccharolobus solfataricus |
5.5 | - |
assay at | Saccharolobus solfataricus |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Saccharolobus solfataricus | isoelectric focusing | - |
4.8 |