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Literature summary for 3.2.1.141 extracted from
- Reaction mechanism of a new glycosyltrehalose-hydrolyzing enzyme isolated from the hyperthermophilic archaeum, Sulfolobus solfataricus KM1 (1996), Biosci. Biotechnol. Biochem., 60, 925-928.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| CuSO4 | - |
Saccharolobus solfataricus |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 4.7 | - |
maltosylpentaosyltrehalose | pH 5.5, 60°C | Saccharolobus solfataricus | |
| 5.6 | - |
maltosyltetraosyltrehalose | pH 5.5, 60°C | Saccharolobus solfataricus | |
| 5.8 | - |
maltosyltriosyltrehalose | pH 5.5, 60°C | Saccharolobus solfataricus | |
| 22.4 | - |
maltosyltrehalose | pH 5.5, 60°C | Saccharolobus solfataricus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 61000 | - |
x * 61000, SDS-PAGE | Saccharolobus solfataricus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Saccharolobus solfataricus | Q55088 | - |
- |
| Saccharolobus solfataricus KM1 | Q55088 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Saccharolobus solfataricus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| maltosylpentaosyltrehalose + H2O | 93.4% of the activity compared to maltosyltetraosyltrehalose, hydrolyzes glycosyltrehaloses from the side of the reducing end only by cleavage of the alpha-1,4 glucosidic bond adjacent to the alpha-1,1 glucosidic bond to liberate trehalose | Saccharolobus solfataricus | trehalose + maltopentaose | - |
? | |
| maltosylpentaosyltrehalose + H2O | 93.4% of the activity compared to maltosyltetraosyltrehalose, hydrolyzes glycosyltrehaloses from the side of the reducing end only by cleavage of the alpha-1,4 glucosidic bond adjacent to the alpha-1,1 glucosidic bond to liberate trehalose | Saccharolobus solfataricus KM1 | trehalose + maltopentaose | - |
? | |
| maltosyltetraosyltrehalose + H2O | hydrolyzes glycosyltrehaloses from the side of the reducing end only by cleavage of the alpha-1,4 glucosidic bond adjacent to the alpha-1,1 glucosidic bond to liberate trehalose | Saccharolobus solfataricus | trehalose + maltotetraose | - |
? | |
| maltosyltetraosyltrehalose + H2O | hydrolyzes glycosyltrehaloses from the side of the reducing end only by cleavage of the alpha-1,4 glucosidic bond adjacent to the alpha-1,1 glucosidic bond to liberate trehalose | Saccharolobus solfataricus KM1 | trehalose + maltotetraose | - |
? | |
| maltosyltrehalose + H2O | 14.4% of the activity compared to maltosyltetraosyltrehalose, hydrolyzes glycosyltrehaloses from the side of the reducing end only by cleavage of the alpha-1,4 glucosidic bond adjacent to the alpha-1,1 glucosidic bond to liberate trehalose | Saccharolobus solfataricus | trehalose + maltose | - |
? | |
| maltosyltrehalose + H2O | 14.4% of the activity compared to maltosyltetraosyltrehalose, hydrolyzes glycosyltrehaloses from the side of the reducing end only by cleavage of the alpha-1,4 glucosidic bond adjacent to the alpha-1,1 glucosidic bond to liberate trehalose | Saccharolobus solfataricus KM1 | trehalose + maltose | - |
? | |
| maltosyltriosyltrehalose + H2O | 91.6% of the activity compared to maltosyltetraosyltrehalose, hydrolyzes glycosyltrehaloses from the side of the reducing end only by cleavage of the alpha-1,4 glucosidic bond adjacent to the alpha-1,1 glucosidic bond to liberate trehalose | Saccharolobus solfataricus | trehalose + maltotriose | - |
? | |
| maltosyltriosyltrehalose + H2O | 91.6% of the activity compared to maltosyltetraosyltrehalose, hydrolyzes glycosyltrehaloses from the side of the reducing end only by cleavage of the alpha-1,4 glucosidic bond adjacent to the alpha-1,1 glucosidic bond to liberate trehalose | Saccharolobus solfataricus KM1 | trehalose + maltotriose | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 61000, SDS-PAGE | Saccharolobus solfataricus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
assay at | Saccharolobus solfataricus |
| 70 | 85 | - |
Saccharolobus solfataricus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 85 | - |
6 h, stable | Saccharolobus solfataricus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4.5 | 5.5 | - |
Saccharolobus solfataricus |
| 5.5 | - |
assay at | Saccharolobus solfataricus |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Saccharolobus solfataricus | isoelectric focusing | - |
4.8 |
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