Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21 (DE3) pLysS cells | Nostoc flagelliforme |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
70000 | - |
x * 70000, deduced from amino acid sequence | Nostoc flagelliforme |
75000 | - |
x * 75000, including the hexahistidine domain from the pET22bZ vector, SDS-PAGE | Nostoc flagelliforme |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Nostoc flagelliforme | E1ANS4 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni2+-NTA-agarose column chromatography, gel filtration | Nostoc flagelliforme |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
maltooligosyltrehalose + H2O | - |
Nostoc flagelliforme | maltooligosaccharide + alpha,alpha'-trehalose | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 70000, deduced from amino acid sequence | Nostoc flagelliforme |
? | x * 75000, including the hexahistidine domain from the pET22bZ vector, SDS-PAGE | Nostoc flagelliforme |
Synonyms | Comment | Organism |
---|---|---|
maltooligosyltrehalose trehalohydrolase | - |
Nostoc flagelliforme |
MTH | - |
Nostoc flagelliforme |
Organism | Comment | Expression |
---|---|---|
Nostoc flagelliforme | the expressions of maltooligosyltrehalose trehalohydrolase in the filaments of Nostoc flagelliforme are upregulated significantly under dehydration stress (exposition to air for 24 h), NaCl stress (exposition to 24-175 mM NaCl for 4 h), and high temperature-drought stress (exposition to air at various 4°C, 10°C, 25°C, 37°C, and 42°C, respectively, for 4 h) | up |