Activating Compound | Comment | Organism | Structure |
---|---|---|---|
IPTG | transcription induced by 1 mM | Metallosphaera hakonensis | |
additional information | addition of 4-alpha-glucanotransferase increases the yield of trehalose production from maltooligosaccharide substrates supplying longer maltooligosaccharides that may be better substrates for the sequential reaction catalyzed by MTS and MTH | Metallosphaera hakonensis |
Cloned (Comment) | Organism |
---|---|
DNA construct pRBMhMTH expressed in Escherichia coli BL21(DE3)pLysS | Metallosphaera hakonensis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
63700 | - |
sequence analysis | Metallosphaera hakonensis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Metallosphaera hakonensis | - |
JCM 8857 | - |
Metallosphaera hakonensis JCM 8857 | - |
JCM 8857 | - |
Purification (Comment) | Organism |
---|---|
by Ni2+-NTA-agarose affinity chromatography | Metallosphaera hakonensis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4'-O-(1,4-alpha-D-glucosyl)n-alpha,alpha'-trehalose + H2O | yield of trehalose production is relatively low but may be increased in the presence of alpha-amylase | Metallosphaera hakonensis | (1,4-alpha-D-glucosyl)n + alpha,alpha'-trehalose | - |
? | |
4'-O-(1,4-alpha-D-glucosyl)n-alpha,alpha'-trehalose + H2O | yield of trehalose production is relatively low but may be increased in the presence of alpha-amylase | Metallosphaera hakonensis JCM 8857 | (1,4-alpha-D-glucosyl)n + alpha,alpha'-trehalose | - |
? | |
maltotriosyltrehalose + H2O | - |
Metallosphaera hakonensis | maltotriose + alpha,alpha'-trehalose | - |
? | |
maltotriosyltrehalose + H2O | - |
Metallosphaera hakonensis JCM 8857 | maltotriose + alpha,alpha'-trehalose | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Maltooligosyl trehalose trehalohydrolase | - |
Metallosphaera hakonensis |
MTH | - |
Metallosphaera hakonensis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
- |
Metallosphaera hakonensis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
retainis 80% of the activity for 48 h, activity is gradually abolished at above 85°C | Metallosphaera hakonensis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | - |
- |
Metallosphaera hakonensis |