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Literature summary for 3.2.1.141 extracted from

  • Nakada, T.; Maruta, K.; Mitsuzumi, H.; Kubota, M.; Chaen, H.; Sugimoto, T.; Kurimoto, M.; Tsujisaka, Y.
    Purification and characterization of a novel enzyme, maltooligosyl trehalose trehalohydrolase, from Arthrobacter sp. Q36 (1995), Biosci. Biotechnol. Biochem., 59, 2215-2218.
    View publication on PubMed
Search for more literature for 3.2.1.141

Inhibitors

Inhibitors Comment Organism Structure
Cu2+
-
 Arthrobacter sp.
Fe2+
-
 Arthrobacter sp.
Hg2+
-
 Arthrobacter sp.
Zn2+
-
 Arthrobacter sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2
-
 maltopentaosyl trehalose
-
 Arthrobacter sp.
4.6
-
 maltotriosyl trehalose
-
 Arthrobacter sp.
5.5
-
 maltosyl trehalose
-
 Arthrobacter sp.
7
-
 maltotetraosyl trehalose
-
 Arthrobacter sp.

Organism

Organism UniProt Comment Textmining
Arthrobacter sp.
-
-
-
Arthrobacter sp. O36
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Arthrobacter sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
497
-
-
 Arthrobacter sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
maltopentaosyltrehalose + H2O
-
 Arthrobacter sp. maltopentaose + alpha,alpha'-trehalose
-
 ?
maltopentaosyltrehalose + H2O
-
 Arthrobacter sp. O36 maltopentaose + alpha,alpha'-trehalose
-
 ?
maltosyltrehalose + H2O
-
 Arthrobacter sp. maltose + alpha,alpha'-trehalose
-
 ?
maltosyltrehalose + H2O
-
 Arthrobacter sp. O36 maltose + alpha,alpha'-trehalose
-
 ?
maltotetraosyltrehalose + H2O
-
 Arthrobacter sp. maltotetraose + alpha,alpha'-trehalose
-
 ?
maltotetraosyltrehalose + H2O
-
 Arthrobacter sp. O36 maltotetraose + alpha,alpha'-trehalose
-
 ?
maltotriosyltrehalose + H2O
-
 Arthrobacter sp. maltotriose + alpha,alpha'-trehalose
-
 ?
maltotriosyltrehalose + H2O
-
 Arthrobacter sp. O36 maltotriose + alpha,alpha'-trehalose
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
-
 Arthrobacter sp.

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30 55 30°C: about 75% of maximal activity, 55°C: about 40% of maximal activity Arthrobacter sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
45
-
 60 min, stable up to Arthrobacter sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
-
 Arthrobacter sp.

pH Range

pH Minimum pH Maximum Comment Organism
5 8 pH 5: about 50% of maximal activity, pH 8: about 40% of maximal activity Arthrobacter sp.

pH Stability

pH Stability pH Stability Maximum Comment Organism
5 10 4°C, 24 h, stable Arthrobacter sp.