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Literature summary for 3.2.1.14 extracted from

  • Jankiewicz, U.; Brzezinska, M.S.
    Purification, characterization, and gene cloning of a chitinase from Stenotrophomonas maltophilia N4 (2015), J. Basic Microbiol., 55, 709-717 .
    View publication on PubMed
Search for more literature for 3.2.1.14

Activating Compound

Activating Compound Comment Organism Structure
2-mercaptoethanol 110% activity at 5 mM Stenotrophomonas maltophilia
EDTA 110% activity at 5 mM Stenotrophomonas maltophilia
L-cysteine 105% activity at 1 mM Stenotrophomonas maltophilia
Triton X-100 104% activity at 1 mM Stenotrophomonas maltophilia
Tween 20 105% activity at 5 mM Stenotrophomonas maltophilia
Tween 80 110% activity at 5 mM Stenotrophomonas maltophilia

Inhibitors

Inhibitors Comment Organism Structure
Cu2+ 80% residual activity at 5 mM Stenotrophomonas maltophilia
Hg2+ 10% residual activity at 5 mM Stenotrophomonas maltophilia
NH4+ 30% residual activity at 5 mM Stenotrophomonas maltophilia
phenylmethylsulfonyl fluoride 64% residual activity at 5 mM Stenotrophomonas maltophilia
SDS 25% residual activity at 5 mM Stenotrophomonas maltophilia
Zn2+ 90% residual activity at 5 mM Stenotrophomonas maltophilia

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.2
-
 4-nitrophenyl beta-D-N,N',N''-triacetylchitotrioside at pH 5.6 and 45°C Stenotrophomonas maltophilia
3.9
-
 4-nitrophenyl N,N'-diacetyl-beta-D-chitobioside at pH 5.6 and 45°C Stenotrophomonas maltophilia

Metals/Ions

Metals/Ions Comment Organism Structure
additional information Mg2+ and Ca2+ do not affect the activity of the enzyme Stenotrophomonas maltophilia

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4-nitrophenyl beta-D-N,N',N''-triacetylchitotrioside + H2O Stenotrophomonas maltophilia highest activity 4-nitrophenol + beta-D-N,N',N''-triacetylchitotriose
-
 ?
4-nitrophenyl beta-D-N,N',N''-triacetylchitotrioside + H2O Stenotrophomonas maltophilia N4 highest activity 4-nitrophenol + beta-D-N,N',N''-triacetylchitotriose
-
 ?
4-nitrophenyl N,N'-diacetyl-beta-D-chitobioside + H2O Stenotrophomonas maltophilia
-
 4-nitrophenol + beta-D-N,N'-diacetylchitobiose
-
 ?
chitin flakes + H2O Stenotrophomonas maltophilia 10.8% activity compared to colloidal chitin N-acetyl-beta-D-glucosamine + ?
-
 ?
chitin powder + H2O Stenotrophomonas maltophilia 32.5% activity compared to colloidal chitin N-acetyl-beta-D-glucosamine + ?
-
 ?
chitosan + H2O Stenotrophomonas maltophilia 75% deacetylated chitosan, 83.8% activity compared to colloidal chitin ?
-
 ?
chitosan + H2O Stenotrophomonas maltophilia N4 75% deacetylated chitosan, 83.8% activity compared to colloidal chitin ?
-
 ?
colloidal chitin + H2O Stenotrophomonas maltophilia 100% activity N-acetyl-beta-D-glucosamine + ?
-
 ?
colloidal chitin + H2O Stenotrophomonas maltophilia N4 100% activity N-acetyl-beta-D-glucosamine + ?
-
 ?
glycol chitin + H2O Stenotrophomonas maltophilia 29.8% activity compared to colloidal chitin N-acetyl-beta-D-glucosamine + ?
-
 ?
laminarin + H2O Stenotrophomonas maltophilia the enzyme demonstrates a very limited capability to hydrolyze beta(1->3) and beta(1->6) glycoside bonds in laminarin. 8% activity compared to colloidal chitin ?
-
 ?
laminarin + H2O Stenotrophomonas maltophilia N4 the enzyme demonstrates a very limited capability to hydrolyze beta(1->3) and beta(1->6) glycoside bonds in laminarin. 8% activity compared to colloidal chitin ?
-
 ?
additional information Stenotrophomonas maltophilia the enzyme does not hydrolyze glycol chitosan, carboxymethyl cellulose and 4-nitrophenyl N-acetyl-beta-D-glucosaminide ?
-
 ?
additional information Stenotrophomonas maltophilia N4 the enzyme does not hydrolyze glycol chitosan, carboxymethyl cellulose and 4-nitrophenyl N-acetyl-beta-D-glucosaminide ?
-
 ?

Organism

Organism UniProt Comment Textmining
Stenotrophomonas maltophilia
-
-
-
Stenotrophomonas maltophilia N4
-
-
-

Purification (Commentary)

Purification (Comment) Organism
DEAE column chromatography and Superdex 200 gel filtration Stenotrophomonas maltophilia

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl beta-D-N,N',N''-triacetylchitotrioside + H2O highest activity Stenotrophomonas maltophilia 4-nitrophenol + beta-D-N,N',N''-triacetylchitotriose
-
 ?
4-nitrophenyl beta-D-N,N',N''-triacetylchitotrioside + H2O highest activity Stenotrophomonas maltophilia N4 4-nitrophenol + beta-D-N,N',N''-triacetylchitotriose
-
 ?
4-nitrophenyl N,N'-diacetyl-beta-D-chitobioside + H2O
-
 Stenotrophomonas maltophilia 4-nitrophenol + beta-D-N,N'-diacetylchitobiose
-
 ?
chitin flakes + H2O 10.8% activity compared to colloidal chitin Stenotrophomonas maltophilia N-acetyl-beta-D-glucosamine + ?
-
 ?
chitin powder + H2O 32.5% activity compared to colloidal chitin Stenotrophomonas maltophilia N-acetyl-beta-D-glucosamine + ?
-
 ?
chitosan + H2O 75% deacetylated chitosan, 83.8% activity compared to colloidal chitin Stenotrophomonas maltophilia ?
-
 ?
chitosan + H2O 75% deacetylated chitosan, 83.8% activity compared to colloidal chitin Stenotrophomonas maltophilia N4 ?
-
 ?
colloidal chitin + H2O 100% activity Stenotrophomonas maltophilia N-acetyl-beta-D-glucosamine + ?
-
 ?
colloidal chitin + H2O 100% activity Stenotrophomonas maltophilia N4 N-acetyl-beta-D-glucosamine + ?
-
 ?
glycol chitin + H2O 29.8% activity compared to colloidal chitin Stenotrophomonas maltophilia N-acetyl-beta-D-glucosamine + ?
-
 ?
laminarin + H2O the enzyme demonstrates a very limited capability to hydrolyze beta(1->3) and beta(1->6) glycoside bonds in laminarin. 8% activity compared to colloidal chitin Stenotrophomonas maltophilia ?
-
 ?
laminarin + H2O the enzyme demonstrates a very limited capability to hydrolyze beta(1->3) and beta(1->6) glycoside bonds in laminarin. 8% activity compared to colloidal chitin Stenotrophomonas maltophilia N4 ?
-
 ?
additional information the enzyme does not hydrolyze glycol chitosan, carboxymethyl cellulose and 4-nitrophenyl N-acetyl-beta-D-glucosaminide Stenotrophomonas maltophilia ?
-
 ?
additional information the enzyme does not hydrolyze glycol chitosan, carboxymethyl cellulose and 4-nitrophenyl N-acetyl-beta-D-glucosaminide Stenotrophomonas maltophilia N4 ?
-
 ?

Subunits

Subunits Comment Organism
? x * 50000, SDS-PAGE Stenotrophomonas maltophilia

Synonyms

Synonyms Comment Organism
endochitinase
-
 Stenotrophomonas maltophilia

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
-
 Stenotrophomonas maltophilia

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
30 55 more than 60% activity between 30 and 55°C Stenotrophomonas maltophilia

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
45
-
 the enzyme demonstrates thermal stability with 80% activity remaining at 45°C within 2 h Stenotrophomonas maltophilia

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.6
-
-
 Stenotrophomonas maltophilia

pH Range

pH Minimum pH Maximum Comment Organism
4.4 7.8 more than 60% activity between pH 4.4 and 7.8 Stenotrophomonas maltophilia