Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ag+ | 37% residual activity at 1 mM | Paenibacillus barengoltzii | |
Ba2+ | 84% residual activity at 1 mM | Paenibacillus barengoltzii | |
Co2+ | 78% residual activity at 1 mM | Paenibacillus barengoltzii | |
Cr2+ | 56% residual activity at 1 mM | Paenibacillus barengoltzii | |
Fe3+ | 83% residual activity at 1 mM | Paenibacillus barengoltzii | |
Hg2+ | 35% residual activity at 1 mM | Paenibacillus barengoltzii | |
Mn2+ | 80% residual activity at 1 mM | Paenibacillus barengoltzii | |
additional information | EDTA and SDS show no significant effects on chitinase activity; EDTA and SDS show no significant effects on enzyme activity | Paenibacillus barengoltzii | |
Ni2+ | 52% residual activity at 1 mM | Paenibacillus barengoltzii | |
Sn2+ | 80% residual activity at 1 mM | Paenibacillus barengoltzii |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 116% activity at 1 mM | Paenibacillus barengoltzii | |
Mg2+ | 124% activity at 1 mM | Paenibacillus barengoltzii | |
additional information | Cu2+, Zn2+ and K+ show no significant effects on chitinase activity | Paenibacillus barengoltzii | |
additional information | Cu2+, Zn2+ and K+ show no significant effects on enzyme activity | Paenibacillus barengoltzii | |
Na+ | 115% activity at 1 mM | Paenibacillus barengoltzii |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
67900 | - |
gel filtration | Paenibacillus barengoltzii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Paenibacillus barengoltzii | A0A0F6NWE3 | - |
- |
Paenibacillus barengoltzii CAU904 | A0A0F6NWE3 | - |
- |
Purification (Comment) | Organism |
---|---|
Q sepharose column chromatography | Paenibacillus barengoltzii |
Q Sepharose column chromatography, and Superdex 200 gel filtration | Paenibacillus barengoltzii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
alpha-chitin + H2O | 2.1% activity compared to colloidal chitin | Paenibacillus barengoltzii | ? | - |
? | |
beta-chitin + H2O | 4.3% activity compared to colloidal chitin | Paenibacillus barengoltzii | ? | - |
? | |
beta-D-N,N',N'',N''',N''''-pentaacetylchitopentaose + 2 H2O | - |
Paenibacillus barengoltzii | 2 beta-D-N,N'-diacetylchitobiose + N-acetyl-beta-D-glucosamine | - |
? | |
beta-D-N,N',N'',N'''-tetraacetylchitotetraose + H2O | - |
Paenibacillus barengoltzii | beta-D-N,N'-diacetylchitobiose + N-acetyl-beta-D-glucosamine | - |
? | |
beta-D-N,N',N''-triacetylchitotriose + H2O | - |
Paenibacillus barengoltzii | beta-D-N,N'-diacetylchitobiose + N-acetyl-beta-D-glucosamine | - |
? | |
carboxymethyl cellulose + H2O | 3.5% activity compared to colloidal chitin | Paenibacillus barengoltzii | ? | - |
? | |
carboxymethyl cellulose + H2O | 3.5% activity compared to colloidal chitin | Paenibacillus barengoltzii CAU904 | ? | - |
? | |
colloidal chitin + H2O | 100% activity | Paenibacillus barengoltzii | beta-D-N,N'-diacetylchitobiose + beta-D-N,N',N''-triacetylchitotriose + beta-D-N,N',N'',N'''-tetraacetylchitotetraose | - |
? | |
colloidal chitin + H2O | 100% activity | Paenibacillus barengoltzii | N-acetyl-beta-D-glucosamine + N,N'-diacetyl-beta-D-chitobiose + N,N',N''-triacetyl-beta-D-chitotriose + tetra-N-acetyl-beta-D-glucosamine | - |
? | |
glycol chitin + H2O | 89.4% activity compared to colloidal chitin | Paenibacillus barengoltzii | ? | - |
? | |
glycol chitin + H2O | 89.4% activity compared to colloidal chitin | Paenibacillus barengoltzii CAU904 | ? | - |
? | |
glycol chitin + H2O | 89.4% activity compared to colloidal chitin | Paenibacillus barengoltzii | N-acetyl-beta-D-glucosamine + ? | - |
? | |
additional information | no or trace activity with N,N'-diacetyl-beta-D-chitobiose, powdery chitin, soluble starch, and 4-nitrophenyl 2-acetamido-2-deoxy-beta-glucopyranoside | Paenibacillus barengoltzii | ? | - |
? | |
additional information | the enzyme shows almost no activity on beta-D-N,N'-diacetylchitobiose | Paenibacillus barengoltzii | ? | - |
? | |
additional information | the enzyme shows no activity with chitosan, soluble starch and 4-nitrophenyl-2-acetamido-2-deoxy-beta-glucopyranoside | Paenibacillus barengoltzii | ? | - |
? | |
additional information | no or trace activity with N,N'-diacetyl-beta-D-chitobiose, powdery chitin, soluble starch, and 4-nitrophenyl 2-acetamido-2-deoxy-beta-glucopyranoside | Paenibacillus barengoltzii CAU904 | ? | - |
? | |
additional information | the enzyme shows almost no activity on beta-D-N,N'-diacetylchitobiose | Paenibacillus barengoltzii CAU904 | ? | - |
? | |
additional information | the enzyme shows no activity with chitosan, soluble starch and 4-nitrophenyl-2-acetamido-2-deoxy-beta-glucopyranoside | Paenibacillus barengoltzii CAU904 | ? | - |
? | |
N,N',N'',N''',N''''-pentaacetyl-beta-D-chitopentaose + H2O | - |
Paenibacillus barengoltzii | N,N',N''-triacetyl-beta-D-chitotriose + N,N'-diacetyl-beta-D-chitobiose + N-acetyl-beta-D-glucosamine | - |
? | |
N,N',N'',N'''-tetraacetyl-beta-D-chitotetraose + H2O | - |
Paenibacillus barengoltzii | N,N'-diacetyl-beta-D-chitobiose + N-acetyl-beta-D-glucosamine | - |
? | |
N,N',N''-triacetyl-beta-D-chitotriose + H2O | - |
Paenibacillus barengoltzii | N,N'-diacetyl-beta-D-chitobiose + N-acetyl-beta-D-glucosamine | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 67000, SDS-PAGE | Paenibacillus barengoltzii |
Synonyms | Comment | Organism |
---|---|---|
acidic chitinase | - |
Paenibacillus barengoltzii |
Chi67 | - |
Paenibacillus barengoltzii |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
- |
Paenibacillus barengoltzii |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | 65 | the enzyme is stable up to 55°C with a thermal denaturing half-life of 43, 102 and 658 min at 65°C, 60°C, and 55°C, respectively | Paenibacillus barengoltzii |
55 | 65 | the enzyme os stable at temperatures below 55°C, since more than 85% of its original activities are retained after incubation at different temperatures for 30 min. The thermal denaturation half-lives of the enzyme at 55, 60 and 65°C are 658, 102 and 43 min, respectively | Paenibacillus barengoltzii |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.016 | - |
glycol chitin | at pH 3.5 and 60°C | Paenibacillus barengoltzii | |
0.019 | - |
colloidal chitin | at pH 3.5 and 60°C | Paenibacillus barengoltzii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
3.5 | - |
- |
Paenibacillus barengoltzii |
3.5 | - |
in 50 mM phosphate-citrate buffer | Paenibacillus barengoltzii |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
3 | 9 | more than 80% activity between pH 3.0 and 9.0 | Paenibacillus barengoltzii |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
3 | 9 | the enzyme displays broad pH stability range in pH 3.0-9.0 as more than 80% of its activities are maintained after 30 min incubation | Paenibacillus barengoltzii |