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Literature summary for 3.2.1.14 extracted from

  • Juarez-Hernandez, E.O.; Casados-Vazquez, L.E.; Bideshi, D.K.; Salcedo-Hernandez, R.; Barboza-Corona, J.E.
    Role of the C-terminal and chitin insertion domains on enzymatic activity of endochitinase ChiA74 of Bacillus thuringiensis (2017), Int. J. Biol. Macromol., 102, 52-59 .
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Rosetta 2 cells Bacillus thuringiensis

Inhibitors

Inhibitors Comment Organism Structure
Ca2+ about 30% residual activity at 1 mM Bacillus thuringiensis
Mg2+ about 30% residual activity at 1 mM Bacillus thuringiensis
Mn2+ about 30% residual activity at 1 mM Bacillus thuringiensis
Zn2+ about 30% residual activity at 1 mM Bacillus thuringiensis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.99
-
4-methylumbelliferyl beta-D-N,N',N''-triacetylchitotrioside mature enzyme lacking its signal peptide sequence, at pH 7.0 and 40°C Bacillus thuringiensis
9.66
-
colloidal chitin mature enzyme lacking its signal peptide sequence, at pH 7.0 and 40°C Bacillus thuringiensis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
colloidal chitin + H2O Bacillus thuringiensis
-
?
-
?

Organism

Organism UniProt Comment Textmining
Bacillus thuringiensis Q93AD3
-
-

Purification (Commentary)

Purification (Comment) Organism
HiTrap Ni affinity column chromatography Bacillus thuringiensis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-methylumbelliferyl beta-D-N,N',N''-triacetylchitotrioside + H2O
-
Bacillus thuringiensis ?
-
?
colloidal chitin + H2O
-
Bacillus thuringiensis ?
-
?

Subunits

Subunits Comment Organism
? x * 70000, mature enzyme lacking its signal peptide sequence, SDS-PAGE Bacillus thuringiensis

Synonyms

Synonyms Comment Organism
ChiA74
-
Bacillus thuringiensis
endochitinase
-
Bacillus thuringiensis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
Bacillus thuringiensis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.24
-
colloidal chitin mature enzyme lacking its signal peptide sequence, at pH 7.0 and 40°C Bacillus thuringiensis
4.98
-
4-methylumbelliferyl beta-D-N,N',N''-triacetylchitotrioside mature enzyme lacking its signal peptide sequence, at pH 7.0 and 40°C Bacillus thuringiensis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
Bacillus thuringiensis

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.43
-
colloidal chitin mature enzyme lacking its signal peptide sequence, at pH 7.0 and 40°C Bacillus thuringiensis
1.66
-
4-methylumbelliferyl beta-D-N,N',N''-triacetylchitotrioside mature enzyme lacking its signal peptide sequence, at pH 7.0 and 40°C Bacillus thuringiensis