Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli Rosetta 2 cells | Bacillus thuringiensis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | about 30% residual activity at 1 mM | Bacillus thuringiensis | |
Mg2+ | about 30% residual activity at 1 mM | Bacillus thuringiensis | |
Mn2+ | about 30% residual activity at 1 mM | Bacillus thuringiensis | |
Zn2+ | about 30% residual activity at 1 mM | Bacillus thuringiensis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.99 | - |
4-methylumbelliferyl beta-D-N,N',N''-triacetylchitotrioside | mature enzyme lacking its signal peptide sequence, at pH 7.0 and 40°C | Bacillus thuringiensis | |
9.66 | - |
colloidal chitin | mature enzyme lacking its signal peptide sequence, at pH 7.0 and 40°C | Bacillus thuringiensis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
colloidal chitin + H2O | Bacillus thuringiensis | - |
? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus thuringiensis | Q93AD3 | - |
- |
Purification (Comment) | Organism |
---|---|
HiTrap Ni affinity column chromatography | Bacillus thuringiensis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-methylumbelliferyl beta-D-N,N',N''-triacetylchitotrioside + H2O | - |
Bacillus thuringiensis | ? | - |
? | |
colloidal chitin + H2O | - |
Bacillus thuringiensis | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 70000, mature enzyme lacking its signal peptide sequence, SDS-PAGE | Bacillus thuringiensis |
Synonyms | Comment | Organism |
---|---|---|
ChiA74 | - |
Bacillus thuringiensis |
endochitinase | - |
Bacillus thuringiensis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
- |
Bacillus thuringiensis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
4.24 | - |
colloidal chitin | mature enzyme lacking its signal peptide sequence, at pH 7.0 and 40°C | Bacillus thuringiensis | |
4.98 | - |
4-methylumbelliferyl beta-D-N,N',N''-triacetylchitotrioside | mature enzyme lacking its signal peptide sequence, at pH 7.0 and 40°C | Bacillus thuringiensis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Bacillus thuringiensis |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.43 | - |
colloidal chitin | mature enzyme lacking its signal peptide sequence, at pH 7.0 and 40°C | Bacillus thuringiensis | |
1.66 | - |
4-methylumbelliferyl beta-D-N,N',N''-triacetylchitotrioside | mature enzyme lacking its signal peptide sequence, at pH 7.0 and 40°C | Bacillus thuringiensis |