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Literature summary for 3.2.1.14 extracted from

  • Yang, S.; Fu, X.; Yan, Q.; Guo, Y.; Liu, Z.; Jiang, Z.
    Cloning, expression, purification and application of a novel chitinase from a thermophilic marine bacterium Paenibacillus barengoltzii (2016), Food Chem., 192, 1041-1048 .
    View publication on PubMed
Search for more literature for 3.2.1.14

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Paenibacillus barengoltzii

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
72300
-
 gel filtration Paenibacillus barengoltzii

Organism

Organism UniProt Comment Textmining
Paenibacillus barengoltzii A0A0F6NWE3
-
-
Paenibacillus barengoltzii CAU904 A0A0F6NWE3
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-IDA column chromatography Paenibacillus barengoltzii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
beta-D-N,N',N'',N''',N''''-pentaacetylchitopentaose + H2O best substrate Paenibacillus barengoltzii beta-D-N,N',N''-triacetylchitotriose + beta-D-N,N'-diacetylchitobiose
-
 ?
beta-D-N,N',N'',N''',N''''-pentaacetylchitopentaose + H2O best substrate Paenibacillus barengoltzii CAU904 beta-D-N,N',N''-triacetylchitotriose + beta-D-N,N'-diacetylchitobiose
-
 ?
beta-D-N,N',N'',N'''-tetraacetylchitotetraose + H2O
-
 Paenibacillus barengoltzii 2 beta-D-N,N'-diacetylchitobiose
-
 ?
beta-D-N,N',N'',N'''-tetraacetylchitotetraose + H2O
-
 Paenibacillus barengoltzii CAU904 2 beta-D-N,N'-diacetylchitobiose
-
 ?
beta-D-N,N',N''-triacetylchitotriose + H2O
-
 Paenibacillus barengoltzii beta-D-N,N'-diacetylchitobiose + N-acetyl-beta-D-glucosamine
-
 ?
colloidal chitin + H2O high activity Paenibacillus barengoltzii beta-D-N,N'-diacetylchitobiose + beta-D-N,N',N''-triacetylchitotriose + beta-D-N,N',N'',N'''-tetraacetylchitotetraose
-
 ?
glycol chitin + H2O
-
 Paenibacillus barengoltzii ?
-
 ?
glycol chitin + H2O
-
 Paenibacillus barengoltzii CAU904 ?
-
 ?
additional information no activity towards beta-D-N,N'-diacetylchitobiose, 4-nitrophenyl N-acetylglucosamine, carboxmethyl cellulose and chitosan Paenibacillus barengoltzii ?
-
 ?
additional information no activity towards beta-D-N,N'-diacetylchitobiose, 4-nitrophenyl N-acetylglucosamine, carboxmethyl cellulose and chitosan Paenibacillus barengoltzii CAU904 ?
-
 ?
powdery chitin + H2O
-
 Paenibacillus barengoltzii ?
-
 ?
powdery chitin + H2O
-
 Paenibacillus barengoltzii CAU904 ?
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 70000, SDS-PAGE Paenibacillus barengoltzii
monomer 1 * 70100, calculated from amino acid sequence Paenibacillus barengoltzii

Synonyms

Synonyms Comment Organism
Chi70
-
 Paenibacillus barengoltzii

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
55
-
-
 Paenibacillus barengoltzii

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
55 65 the half-lives of the enzyme at 55, 60, and 65°C are 323, 97, and 29 min, respectively Paenibacillus barengoltzii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5
-
-
 Paenibacillus barengoltzii

pH Stability

pH Stability pH Stability Maximum Comment Organism
4 9.5 the enzyme retains more than 80% of its maximal activity after 30 min at pH 4.0-9.5 Paenibacillus barengoltzii

pI Value

Organism Comment pI Value Maximum pI Value
Paenibacillus barengoltzii calculated from amino acid sequence
-
 5.7