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Literature summary for 3.2.1.14 extracted from

  • Nguyen, H.; Nguyen, T.; Nguyen, T.; Peterbauer, C.; Mathiesen, G.; Haltrich, D.
    Chitinase from Bacillus licheniformis DSM13: expression in Lactobacillus plantarum WCFS1 and biochemical characterisation (2012), Protein Expr. Purif., 81, 166-174.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
gene chi, cloning in Escherichia coli strain NEB 5alpha, recombinant expression of His-tagged enzyme in Lactobacillus plantarum strain WCFS1 using inducible lactobacillal expression vectors pSIP403 and pSIP409, derived from the sakacin-P operon of Lactobacillus sakei Bacillus licheniformis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
65000
-
1 * 65000, recombinant His-tagged enzyme, SDS-PAGE Bacillus licheniformis
66500
-
recombinant His-tagged enzyme, gel filtration Bacillus licheniformis

Organism

Organism UniProt Comment Textmining
Bacillus licheniformis A5A699 gene CHI
-
Bacillus licheniformis DSM 13 A5A699 gene CHI
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged enzyme from Lactobacillus plantarum strain WCFS1 by immobilized metal affinity chromatography Bacillus licheniformis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
chitin + H2O from crab shells Bacillus licheniformis ?
-
?
chitin + H2O from crab shells Bacillus licheniformis DSM 13 ?
-
?
chitosan + H2O
-
Bacillus licheniformis ?
-
?
chitosan + H2O
-
Bacillus licheniformis DSM 13 ?
-
?
N,N'-diacetylchitobiose + H2O
-
Bacillus licheniformis deacetylated chitooligosaccharides + acetate
-
?
N,N'-diacetylchitobiose + H2O
-
Bacillus licheniformis DSM 13 deacetylated chitooligosaccharides + acetate
-
?

Subunits

Subunits Comment Organism
monomer 1 * 65000, recombinant His-tagged enzyme, SDS-PAGE Bacillus licheniformis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
recombinant His-tagged enzyme Bacillus licheniformis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
37
-
purified recombinant enzyme, half-life is 20 days Bacillus licheniformis
50
-
purified recombinant enzyme, half-life is 8.4 days Bacillus licheniformis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
recombinant His-tagged enzyme Bacillus licheniformis

pH Stability

pH Stability pH Stability Maximum Comment Organism
5 10 purified recombinant enzyme, stable at, over 80% activity remaining Bacillus licheniformis

General Information

General Information Comment Organism
evolution the enzyme belongs to the glycosyl hydrolase family GH18 Bacillus licheniformis