Cloned (Comment) | Organism |
---|---|
gene chi, cloning in Escherichia coli strain NEB 5alpha, recombinant expression of His-tagged enzyme in Lactobacillus plantarum strain WCFS1 using inducible lactobacillal expression vectors pSIP403 and pSIP409, derived from the sakacin-P operon of Lactobacillus sakei | Bacillus licheniformis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
65000 | - |
1 * 65000, recombinant His-tagged enzyme, SDS-PAGE | Bacillus licheniformis |
66500 | - |
recombinant His-tagged enzyme, gel filtration | Bacillus licheniformis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus licheniformis | A5A699 | gene CHI | - |
Bacillus licheniformis DSM 13 | A5A699 | gene CHI | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from Lactobacillus plantarum strain WCFS1 by immobilized metal affinity chromatography | Bacillus licheniformis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
chitin + H2O | from crab shells | Bacillus licheniformis | ? | - |
? | |
chitin + H2O | from crab shells | Bacillus licheniformis DSM 13 | ? | - |
? | |
chitosan + H2O | - |
Bacillus licheniformis | ? | - |
? | |
chitosan + H2O | - |
Bacillus licheniformis DSM 13 | ? | - |
? | |
N,N'-diacetylchitobiose + H2O | - |
Bacillus licheniformis | deacetylated chitooligosaccharides + acetate | - |
? | |
N,N'-diacetylchitobiose + H2O | - |
Bacillus licheniformis DSM 13 | deacetylated chitooligosaccharides + acetate | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 65000, recombinant His-tagged enzyme, SDS-PAGE | Bacillus licheniformis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
recombinant His-tagged enzyme | Bacillus licheniformis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
purified recombinant enzyme, half-life is 20 days | Bacillus licheniformis |
50 | - |
purified recombinant enzyme, half-life is 8.4 days | Bacillus licheniformis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
recombinant His-tagged enzyme | Bacillus licheniformis |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
5 | 10 | purified recombinant enzyme, stable at, over 80% activity remaining | Bacillus licheniformis |
General Information | Comment | Organism |
---|---|---|
evolution | the enzyme belongs to the glycosyl hydrolase family GH18 | Bacillus licheniformis |