Cloned (Comment) | Organism |
---|---|
gene VvChi4D, DNA and amino acid sequence determination and analysis | Vitis vinifera |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
31000 | - |
x * 31000, SDS-PAGE | Vitis vinifera |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
chitin + H2O | Vitis vinifera | chitinases are glycosyl hydrolases that catalyze the degradation of chitin, a beta-1,4-linked polymer of N-acetylglucosamine | N,N'-diacetylchitobiose + ? | - |
? | |
chitin + H2O | Vitis vinifera Manzoni Bianco | chitinases are glycosyl hydrolases that catalyze the degradation of chitin, a beta-1,4-linked polymer of N-acetylglucosamine | N,N'-diacetylchitobiose + ? | - |
? | |
N-acetylglucosamine oligomers + H2O | Vitis vinifera | - |
N-acetylglucosamine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Vitis vinifera | Q7XAU6 | gene VvChi4D | - |
Vitis vinifera Manzoni Bianco | Q7XAU6 | gene VvChi4D | - |
Purification (Comment) | Organism |
---|---|
native enzyme from filtered grape juice in a procedure including cation exchange chromatography and dialysis | Vitis vinifera |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
fruit | grape juice, type IV grape chitinase | Vitis vinifera | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
chitin + H2O | crab shell chitin | Vitis vinifera | ? | - |
? | |
chitin + H2O | crab shell chitin | Vitis vinifera Manzoni Bianco | ? | - |
? | |
chitin + H2O | chitinases are glycosyl hydrolases that catalyze the degradation of chitin, a beta-1,4-linked polymer of N-acetylglucosamine | Vitis vinifera | N,N'-diacetylchitobiose + ? | - |
? | |
chitin + H2O | chitinases are glycosyl hydrolases that catalyze the degradation of chitin, a beta-1,4-linked polymer of N-acetylglucosamine | Vitis vinifera Manzoni Bianco | N,N'-diacetylchitobiose + ? | - |
? | |
chitohexaose + H2O | reaction via trimer formation | Vitis vinifera | 2 N-acetylglucosamine + 2 N,N'-diacetylchitobiose | - |
? | |
chitopentaose + H2O | reaction via trimer formation | Vitis vinifera | N-acetylglucosamine + 2 N,N'-diacetylchitobiose | - |
? | |
colloidal chitin + H2O | - |
Vitis vinifera | N,N'-diacetylchitobiose + ? | the molecule is not further processed, absence of 1,4-beta-N-acetylglucosaminidase activity | ? | |
colloidal chitin + H2O | - |
Vitis vinifera Manzoni Bianco | N,N'-diacetylchitobiose + ? | the molecule is not further processed, absence of 1,4-beta-N-acetylglucosaminidase activity | ? | |
additional information | type IV enzyme exhibits both endo- and exochitinase activities, absence of 1,4-beta-N-acetylglucosaminidase activity | Vitis vinifera | ? | - |
? | |
additional information | type IV enzyme exhibits both endo- and exochitinase activities, absence of 1,4-beta-N-acetylglucosaminidase activity | Vitis vinifera Manzoni Bianco | ? | - |
? | |
N,N',N'',N'''-tetraacetylchitotetraose + H2O | reaction via trimer formation | Vitis vinifera | 2 N-acetylglucosamine + N,N'-diacetylchitobiose | - |
? | |
N,N',N''-triacetylchitotriose + H2O | - |
Vitis vinifera | N-acetyl-D-glucosamine + N,N'-diacetylchitobiose | - |
? | |
N,N',N''-triacetylchitotriose + H2O | - |
Vitis vinifera Manzoni Bianco | N-acetyl-D-glucosamine + N,N'-diacetylchitobiose | - |
? | |
N-acetylglucosamine oligomers + H2O | - |
Vitis vinifera | N-acetylglucosamine | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 31000, SDS-PAGE | Vitis vinifera |
More | tryptic peptide mapping and mass spectrometric analysis | Vitis vinifera |
Synonyms | Comment | Organism |
---|---|---|
class IV chitinase | - |
Vitis vinifera |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
- |
Vitis vinifera |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
10 | 60 | activity range, at above 50°C, activity decreases drastically, profile overview | Vitis vinifera |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
Tm at pH 4.0 | Vitis vinifera |
70 | - |
purified enzyme, pH 6.0, 1 h, 30% activity remaining | Vitis vinifera |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
- |
Vitis vinifera |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 6.5 | narrow pH spectrum, sharp decrease in activity below and above | Vitis vinifera |
General Information | Comment | Organism |
---|---|---|
evolution | the enzyme belongs to carbohydrate-binding module family 18 and glycoside hydrolase family GH19. Possibly the two proteins class IV chitinase and class IV endochitinase, UniProt IDs Q7XAU6 and O24530, are allelic variants encoded by the same gene | Vitis vinifera |
additional information | at a concentration and pH expected in ripe grapes, no inhibition of wine yeast growth by the chitinase is observed | Vitis vinifera |