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Literature summary for 3.2.1.14 extracted from
- Production and purification of a hyperthermostable chitinase from Brevibacillus formosus BISR-1 isolated from the Great Indian Desert soils (2014), Extremophiles, 18, 451-462.
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | the enzyme is secreted | Brevibacillus formosus | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 37600 | - |
x * 37600 | Brevibacillus formosus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| chitin + H2O | Brevibacillus formosus | - |
? | - |
? |  |
| chitin + H2O | Brevibacillus formosus BISR-1 | - |
? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Brevibacillus formosus | - |
isolated from the Great Indian Desert soils | - |
| Brevibacillus formosus BISR-1 | - |
isolated from the Great Indian Desert soils | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| native extracellular enzyme 6.1fold from culture supernatant by ultrafiltration, ammonium sulfate fractionation, and adsorption chromatography | Brevibacillus formosus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| additional information | high level enzyme expression in strain BISR-1, optimized conditions with medium containing chitin 2.0 g/l, malt extract 1.5 g/l, glycerol 1.0 g/l, ammonium nitrate 0.3%, T-20 0.1%, pH 7.0, 37°C, optimization process, detailed overview. Temperature growth limit is 50°C, sharp decrase in enzyme activity at 45°C. Effects of surfactants on enzyme production, overview | Brevibacillus formosus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
203 U/ml, pH 7.0, 37°C, optimized enzyme production conditions | Brevibacillus formosus |
| 94.21 | - |
purified enzyme, pH 7.0, 37°C | Brevibacillus formosus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| chitin + H2O | - |
Brevibacillus formosus | ? | - |
? | |
| chitin + H2O | - |
Brevibacillus formosus BISR-1 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 37600 | Brevibacillus formosus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 55 | - |
- |
Brevibacillus formosus |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 35 | 100 | activity range | Brevibacillus formosus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 80 | - |
purified native enzyme, 60% activity remaining after 72 h | Brevibacillus formosus |
| 90 | - |
purified native enzyme, half-life is 72 h | Brevibacillus formosus |
| 100 | - |
purified native enzyme, half-life is 5 h | Brevibacillus formosus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5 | - |
- |
Brevibacillus formosus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 3 | 10 | activity range | Brevibacillus formosus |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 10 | the purified enzyme retains over 65% residual activity in this range | Brevibacillus formosus |
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