Cloned (Comment) | Organism |
---|---|
recombinant expression of His6-tagged enzyme in Escherichia coli strain BL21(DE3) | Nepenthes alata |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Michaelis-Menten kinetics, 1.49 mg/ml ethlene glycol chitin at pH 3.9, 37°C, and 1.25 mg/ml chitohexaose at pH 3.9, 37°C | Nepenthes alata |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
chitin + H2O | Nepenthes alata | chitinase catalyzes the hydrolysis of a beta-1,4 glycosidic bond in chitin which is a linear polysaccharide of N-acetyl-beta-D-glucosamine | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Nepenthes alata | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity and anion exchange chromatography | Nepenthes alata |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
pitcher | - |
Nepenthes alata | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
chitin + H2O | chitinase catalyzes the hydrolysis of a beta-1,4 glycosidic bond in chitin which is a linear polysaccharide of N-acetyl-beta-D-glucosamine | Nepenthes alata | ? | - |
? | |
chitohexaose + H2O | the enzyme hydrolyzed the fourth glycosidic linkage from the non-reducing end of (GlcNAc)6 | Nepenthes alata | chitotetraose + chitobiose | - |
? | |
chitopentaose + H2O | - |
Nepenthes alata | chitotetraose + N-acetyl-beta-D-glucosamine | - |
? | |
colloidal chitin + H2O | - |
Nepenthes alata | ? | - |
? | |
ethlene glycol chitin + H2O | - |
Nepenthes alata | ? | - |
? | |
additional information | the enzyme is a a retaining enzyme. No activity with chitotetraose. Anomeric forms of the reaction products | Nepenthes alata | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
NaCHIT3 | - |
Nepenthes alata |
plant class III chitinase | - |
Nepenthes alata |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
65 | - |
- |
Nepenthes alata |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.1 | - |
ethlene glycol chitin | pH 3.9, 37°C | Nepenthes alata | |
0.68 | - |
chitohexaose | pH 3.9, 37°C | Nepenthes alata |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
3.9 | - |
- |
Nepenthes alata |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
3.6 | 6.9 | high activity within this range | Nepenthes alata |
General Information | Comment | Organism |
---|---|---|
evolution | the enzyme belongs to the glycosyl hydrolase family GH18 | Nepenthes alata |
physiological function | plant chitinases are considered to be pathogen related proteins used to defend against invasions such as external fungi and insects attacks, that contain chitins | Nepenthes alata |