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Literature summary for 3.2.1.14 extracted from

  • Ishisaki, K.; Arai, S.; Hamada, T.; Honda, Y.
    Biochemical characterization of a recombinant plant class III chitinase from the pitcher of the carnivorous plant Nepenthes alata (2012), Carbohydr. Res., 361, 170-174.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
recombinant expression of His6-tagged enzyme in Escherichia coli strain BL21(DE3) Nepenthes alata

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information Michaelis-Menten kinetics, 1.49 mg/ml ethlene glycol chitin at pH 3.9, 37°C, and 1.25 mg/ml chitohexaose at pH 3.9, 37°C Nepenthes alata

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
chitin + H2O Nepenthes alata chitinase catalyzes the hydrolysis of a beta-1,4 glycosidic bond in chitin which is a linear polysaccharide of N-acetyl-beta-D-glucosamine ?
-
?

Organism

Organism UniProt Comment Textmining
Nepenthes alata
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity and anion exchange chromatography Nepenthes alata

Source Tissue

Source Tissue Comment Organism Textmining
pitcher
-
Nepenthes alata
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
chitin + H2O chitinase catalyzes the hydrolysis of a beta-1,4 glycosidic bond in chitin which is a linear polysaccharide of N-acetyl-beta-D-glucosamine Nepenthes alata ?
-
?
chitohexaose + H2O the enzyme hydrolyzed the fourth glycosidic linkage from the non-reducing end of (GlcNAc)6 Nepenthes alata chitotetraose + chitobiose
-
?
chitopentaose + H2O
-
Nepenthes alata chitotetraose + N-acetyl-beta-D-glucosamine
-
?
colloidal chitin + H2O
-
Nepenthes alata ?
-
?
ethlene glycol chitin + H2O
-
Nepenthes alata ?
-
?
additional information the enzyme is a a retaining enzyme. No activity with chitotetraose. Anomeric forms of the reaction products Nepenthes alata ?
-
?

Synonyms

Synonyms Comment Organism
NaCHIT3
-
Nepenthes alata
plant class III chitinase
-
Nepenthes alata

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
65
-
-
Nepenthes alata

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.1
-
ethlene glycol chitin pH 3.9, 37°C Nepenthes alata
0.68
-
chitohexaose pH 3.9, 37°C Nepenthes alata

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
3.9
-
-
Nepenthes alata

pH Range

pH Minimum pH Maximum Comment Organism
3.6 6.9 high activity within this range Nepenthes alata

General Information

General Information Comment Organism
evolution the enzyme belongs to the glycosyl hydrolase family GH18 Nepenthes alata
physiological function plant chitinases are considered to be pathogen related proteins used to defend against invasions such as external fungi and insects attacks, that contain chitins Nepenthes alata