Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.2.1.14 extracted from

  • Akond, M.A.; Matsuda, Y.; Ishimaru, T.; Iwai, K.; Saito, A.; Kato, A.; Tanaka, S.; Kobayashi, J.; Koga, D.
    Characterization of a yam class IV chitinase produced by recombinant Pichia pastoris X-33 (2014), Biosci. Biotechnol. Biochem., 78, 222-230.
    View publication on PubMed
Search for more literature for 3.2.1.14

Cloned(Commentary)

Cloned (Comment) Organism
cloning in Escherichia coli strain JM109, removal of the C-terminal VTS of 24 bp and replacement of the N-terminal signal sequence of yam chitinase with alpha-factor of the expression vector pPICZalphaA, recombinant expression in Pichia pastoris strain X-33 an secretion to the culture medium Dioscorea polystachya

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information 0.233 mg/ml at pH 8.0, 32°C, substrate glycol chitin, recombinant enzyme Dioscorea polystachya

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Dioscorea polystachya
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
32000
-
 x * 32000, recombinant deglycosylated enzyme, SDS-PAGE Dioscorea polystachya

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
chitin + H2O Dioscorea polystachya
-
 ?
-
 ?

Organism

Organism UniProt Comment Textmining
Dioscorea polystachya Q852P9
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein deglycosylation by endoglycosidase H Dioscorea polystachya

Purification (Commentary)

Purification (Comment) Organism
recombinant extracellular enzyme 10fold from Pichia pastoris culture supernatant by several different steps of anion exchange chromatography and gel filtration Dioscorea polystachya

Source Tissue

Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.23
-
 purified recombinant enzyme, pH 8.0, 32°C, substrate glycolchitin Dioscorea polystachya

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
chitin + H2O
-
 Dioscorea polystachya ?
-
 ?
glycol chitin + H2O
-
 Dioscorea polystachya ?
-
 ?

Subunits

Subunits Comment Organism
? x * 32000, recombinant deglycosylated enzyme, SDS-PAGE Dioscorea polystachya

Synonyms

Synonyms Comment Organism
class IV chitinase
-
 Dioscorea polystachya

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
70
-
-
 Dioscorea polystachya

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
 purified native deglycosylated enzyme, pH 8.0, 15 min, completely stable Dioscorea polystachya
60
-
 purified native deglycosylated enzyme, pH 8.0, 15 min, over 90% activity remaining Dioscorea polystachya
70
-
 purified native deglycosylated enzyme, pH 8.0, 15 min, over 60% activity remaining Dioscorea polystachya
75
-
 purified native deglycosylated enzyme, pH 8.0, 15 min, 10% activity remaining Dioscorea polystachya

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.2
-
 glycol chitin pH 8.0, 32°C, recombinant enzyme Dioscorea polystachya

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
 main larger peak Dioscorea polystachya
8
-
 second smaller peak Dioscorea polystachya

pH Range

pH Minimum pH Maximum Comment Organism
3 12 activity range, profile overview Dioscorea polystachya

pH Stability

pH Stability pH Stability Maximum Comment Organism
3 12 purified native deglycosylated enzyme, 32°C, completely stable Dioscorea polystachya

General Information

General Information Comment Organism
evolution the enzyme belongs to the glycosyl hydrolase family GH 19 Dioscorea polystachya
physiological function recombinant Pichia pastoris-produced chitinase shows strong lytic activity against Fusarium oxysporum and Phytophthora nicotianae Dioscorea polystachya