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Literature summary for 3.2.1.14 extracted from

  • Babashpour, S.; Aminzadeh, S.; Farrokhi, N.; Karkhane, A.; Haghbeen, K.
    Characterization of a chitinase (Chit62) from Serratia marcescens B4A and its efficacy as a bioshield against plant fungal pathogens (2012), Biochem. Genet., 50, 722-735.
    View publication on PubMed
Search for more literature for 3.2.1.14

Cloned(Commentary)

Cloned (Comment) Organism
DNA and amino acid sequence determination and analysis, phylogenetic tree, recombinant expression of His6-tagged enzyme in Escherichia coli strain BL21 (DE3) Serratia marcescens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information Michaelis-Menten kinetics, 3.30 mg/ml with colloidal chitin, pH 6.0, 50°C, recombinant enzyme Serratia marcescens

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular the enzyme contains a 25 amino acid signal peptide and is secreted Serratia marcescens
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
62000
-
 x * 62000, recombinant His6-tagged enzyme, SDS-PAGE Serratia marcescens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
chitin + H2O Serratia marcescens
-
 ?
-
 ?
chitin + H2O Serratia marcescens B4A
-
 ?
-
 ?

Organism

Organism UniProt Comment Textmining
Serratia marcescens E5FGC5
-
-
Serratia marcescens B4A E5FGC5
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His6-tagged enzyme 5fold from Escherichia coli strain BL21 (DE3) by nickel affinity chromatography and dialysis Serratia marcescens

Reaction

Reaction Comment Organism Reaction ID
chitopentaose + H2O = chitobiose + chitotriose double displacement reaction mechanism, overview Serratia marcescens
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
chitin + H2O
-
 Serratia marcescens ?
-
 ?
chitin + H2O
-
 Serratia marcescens B4A ?
-
 ?
colloidal chitin + H2O
-
 Serratia marcescens ?
-
 ?
colloidal chitin + H2O
-
 Serratia marcescens B4A ?
-
 ?

Subunits

Subunits Comment Organism
? x * 62000, recombinant His6-tagged enzyme, SDS-PAGE Serratia marcescens

Synonyms

Synonyms Comment Organism
Chit62
-
 Serratia marcescens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
55
-
-
 Serratia marcescens

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
15 85 activity range, over 50% activity at 30-60°C Serratia marcescens

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 purified enzyme, pH 6.0, completely stable up to for 20 min Serratia marcescens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
 Serratia marcescens

pH Range

pH Minimum pH Maximum Comment Organism
4 10 activity range, over 50% of maximal activity at pH 5.0-8.0 Serratia marcescens

pH Stability

pH Stability pH Stability Maximum Comment Organism
4 9 purified enzyme, 50°C, 1 h, over 50% activity remaing within this range, completely stable at pH 6.0-8.0 Serratia marcescens

pI Value

Organism Comment pI Value Maximum pI Value
Serratia marcescens sequence calculation
-
 6.35

General Information

General Information Comment Organism
evolution phylogenetic analysis reveals the possibility of horizontal gene transfer between bacteria, viruses, and insects. The Chit62 enzyme is clearly grouped with other bacterial chitinases, but with a clear distinction from previously reported Serratia marcescens Serratia marcescens
physiological function the active enzyme shows biocontrol features against fungal phytopathogens Serratia marcescens