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Literature summary for 3.2.1.14 extracted from
- A novel family 19 chitinase from the marine-derived Pseudoalteromonas tunicata CCUG 44952T: heterologous expression, characterization and antifungal activity (2015), Biochem. Eng. J., 93, 84-93.
No PubMed abstract available
Application
| Application | Comment | Organism |
|---|---|---|
| pharmacology | since the enzyme exhibits antifungal activity against phytopathogenic and human pathogenic fungi, it can be used as an alternative biofungicide | Pseudoalteromonas tunicata |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene Ptchi19, DNA and amino acid sequence determination and analysis, expression in Eschereichia coli | Pseudoalteromonas tunicata |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | 8% inhibition at 5 mM | Pseudoalteromonas tunicata |  |
| Cd2+ | 93% inhibition at 5 mM | Pseudoalteromonas tunicata | |
| Cu2+ | complete inhibition at 5 mM | Pseudoalteromonas tunicata | |
| Fe2+ | 88% inhibition at 5 mM | Pseudoalteromonas tunicata | |
| Hg2+ | complete inhibition at 5 mM | Pseudoalteromonas tunicata | |
| K+ | 10% inhibition at 5 mM | Pseudoalteromonas tunicata | |
| Mn2+ | 34% inhibition at 5 mM | Pseudoalteromonas tunicata | |
| additional information | poor inhibition by Mg2+ at 5 mM | Pseudoalteromonas tunicata | |
| Ni2+ | 89% inhibition at 5 mM | Pseudoalteromonas tunicata | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 53500 | - |
x * 53500, recombinant enzyme, SDS-PAGE | Pseudoalteromonas tunicata |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudoalteromonas tunicata | - |
gene Ptchi19 | - |
| Pseudoalteromonas tunicata CCUG 44952T | - |
gene Ptchi19 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant enzyme 8.2fold from Escherichia coli | Pseudoalteromonas tunicata |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 16.4 | - |
purified recombinant enzyme, pH 7.5, 43°C, substrate crystalline chitin | Pseudoalteromonas tunicata |
| 59.9 | - |
purified recombinant enzyme, pH 7.5, 43°C, substrate colloidal chitin | Pseudoalteromonas tunicata |
| 2382 | - |
purified recombinant enzyme, pH 7.5, 43°C, substrate 4-nitrophenyl N-acetyl-beta-D-glucosamine | Pseudoalteromonas tunicata |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-nitrophenyl N-acetyl-beta-D-glucosamine + H2O | best substrate | Pseudoalteromonas tunicata | 4-nitrophenol + N-acetyl-beta-D-glucosamine | - |
? | |
| 4-nitrophenyl N-acetyl-beta-D-glucosamine + H2O | best substrate | Pseudoalteromonas tunicata CCUG 44952T | 4-nitrophenol + N-acetyl-beta-D-glucosamine | - |
? | |
| colloidal chitin + H2O | from crab shell chitin | Pseudoalteromonas tunicata | ? | - |
? | |
| colloidal chitin + H2O | from crab shell chitin | Pseudoalteromonas tunicata CCUG 44952T | ? | - |
? | |
| crystalline chitin + H2O | from crab shells | Pseudoalteromonas tunicata | ? | - |
? | |
| crystalline chitin + H2O | from crab shells | Pseudoalteromonas tunicata CCUG 44952T | ? | - |
? | |
| additional information | no activity with 4-nitrophenyl N,N',N''-beta-D-triacetylchitotriose and 4-nitrophenyl N,N'-beta-diacetylchitobiose | Pseudoalteromonas tunicata | ? | - |
? | |
| additional information | no activity with 4-nitrophenyl N,N',N''-beta-D-triacetylchitotriose and 4-nitrophenyl N,N'-beta-diacetylchitobiose | Pseudoalteromonas tunicata CCUG 44952T | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 53500, recombinant enzyme, SDS-PAGE | Pseudoalteromonas tunicata |
| More | multi-domain structure are the domain (D132-A155) where the active site is located, and the domain (A437-W479) that includes a C-terminal carbohydrate-binding module 5 | Pseudoalteromonas tunicata |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Ptchi19p | - |
Pseudoalteromonas tunicata |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | - |
- |
Pseudoalteromonas tunicata |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | 50 | recombinant enzyme, activity range | Pseudoalteromonas tunicata |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
assay at | Pseudoalteromonas tunicata |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 9.5 | recombinant enzyme, activity range | Pseudoalteromonas tunicata |
General Information
| General Information | Comment | Organism |
|---|---|---|
| evolution | the enzyme belongs to the glycosyl hydrolase family GH19 | Pseudoalteromonas tunicata |
| physiological function | the enzyme exhibits antifungal activity against phytopathogenic and human pathogenic fungi | Pseudoalteromonas tunicata |
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