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Literature summary for 3.2.1.14 extracted from
- Highly thermostable and surfactant-activated chitinase from a subseafloor bacterium, Laceyella putida (2014), Appl. Microbiol. Biotechnol., 98, 7845-7853.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| benzalkonium chloride | activates at 0.1% w/v by about 60-200% dependent on the substrate, overview | Laceyella putida |  |
| N-hexadecyltrimethylammonium chloride | activates at 0.1% w/v by about 60% dependent on the substrate, overview | Laceyella putida | |
| SDS | activates at 0.1% w/v by about 60-200% dependent on the substrate, overview | Laceyella putida | |
| Triton X-100 | activates at 0.1% w/v by about 60-200% dependent on the substrate, overview | Laceyella putida | |
| Tween 80 | activates at 0.1% w/v by about 60-200% dependent on the substrate, overview | Laceyella putida | |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| DNA and amino acid sequence determination and analysis, sequence comparison | Laceyella putida |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Cu2+ | 35% inhibition at 2.5 mM | Laceyella putida | |
| Fe3+ | 40% inhibition at 2.5 mM | Laceyella putida | |
| Hg2+ | 42% inhibition at 2.5 mM | Laceyella putida | |
| Pd2+ | complete inhibition at 2.5 mM | Laceyella putida | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Laceyella putida | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| additional information | no significant effect by 2.5 mM of Ca2+, Co2+, Mn2+, Zn2+, Mg2+, and Ni2+. 10 mM each EDTA and EGTA, 5 mM each 1-ethyl-3-dimethyl aminopropylcarbadimide, 2-mercaptoethanol, dithiothreitol, N-ethylmaleimide, 1 mM N-bromosuccinimide, and 0.1 mM 4-chloromercuribenzoate do not essentially inhibit the enzyme | Laceyella putida |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 38000 | - |
2 * 38000, SDS-PAGE | Laceyella putida |
| 75000 | - |
gel filtration | Laceyella putida |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Laceyella putida | - |
isolated from subseafloor core sediment at 0.5 mbsf | - |
| Laceyella putida JAM FM3001 | - |
isolated from subseafloor core sediment at 0.5 mbsf | - |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| proteolytic modification | the enzyme is cleaved at C-terminus of Thr176 after translation. Subsequently, the catalytic domain is dimerized to be active | Laceyella putida |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| native extracellular enzyme from cuture meidum of strain JAM FM3001 by ammonium sulfate fractionation, anion exchange chromatography, dialysis, another step of anion exchange chromatography, hydrophobic interaction chromatography, and ultrafiltration, to homogeneity | Laceyella putida |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-nitrophenyl N,N'-diacetylchitobiose + H2O | - |
Laceyella putida | 4-nitrophenol + N,N'-diacetylchitobiose | - |
? | |
| 4-nitrophenyl N,N'-diacetylchitobiose + H2O | - |
Laceyella putida JAM FM3001 | 4-nitrophenol + N,N'-diacetylchitobiose | - |
? | |
| 4-nitrophenyl N-acetyl-D-glucosamine + H2O | - |
Laceyella putida | 4-nitrophenol + N-acetyl-D-glucosamine | - |
? | |
| 4-nitrophenyl N-acetyl-D-glucosamine + H2O | - |
Laceyella putida JAM FM3001 | 4-nitrophenol + N-acetyl-D-glucosamine | - |
? | |
| colloidal chitin + H2O | - |
Laceyella putida | ? | - |
? | |
| colloidal chitin + H2O | - |
Laceyella putida JAM FM3001 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homodimer | 2 * 38000, SDS-PAGE | Laceyella putida |
| More | the enzyme has a chitin-binding domain and a fibronectin type 3-like domain in the N-terminal region, molecular domain structure, comparisons, overview | Laceyella putida |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| LpChiA | - |
Laceyella putida |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 75 | - |
- |
Laceyella putida |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
1 h, 25 mM acetate buffer, pH 5.2, 80% activity remaining after 350 h | Laceyella putida |
| 60 | - |
1 h, 25 mM acetate buffer, pH 5.2, 80% activity remaining after 270 h | Laceyella putida |
| 70 | - |
1 h, 25 mM acetate buffer, pH 5.2, half-life is 3 days, 80% activity remaining after 100 h | Laceyella putida |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4 | - |
- |
Laceyella putida |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 2.5 | 10 | activity range, activity profile overview | Laceyella putida |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 3 | 11 | purified enzyme, 60°C, 1 h, completely stable | Laceyella putida |
Expression
| Organism | Comment | Expression |
|---|---|---|
| Laceyella putida | the enzyme is inducible by adding 0.2% colloidal chitin or or 0.5% N-acetylglucosamine | up |
General Information
| General Information | Comment | Organism |
|---|---|---|
| evolution | the enzyme belongs to the glycosyl hydrolase family GH 18 | Laceyella putida |
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