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Literature summary for 3.2.1.14 extracted from
- Purification and characterization of an antifungal chitinase from Citrobacter freundii str. nov. haritD11 (2014), Appl. Biochem. Biotechnol., 172, 196-205.
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 64000 | - |
x * 64000, SDS-PAGE | Citrobacter freundii |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Citrobacter freundii | - |
str. nov. | - |
| Citrobacter freundii haritD11 | - |
str. nov. | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| native enzyme 7.3fold by ammonium sulfate fractionation, dialysis, and gel filtration | Citrobacter freundii |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 140.6 | - |
purified enzyme, pH 8.0, 35°C, substrate colloidal chitin | Citrobacter freundii |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| colloidal chitin + H2O | - |
Citrobacter freundii | ? | - |
? |  |
| colloidal chitin + H2O | - |
Citrobacter freundii haritD11 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 64000, SDS-PAGE | Citrobacter freundii |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 35 | 40 | - |
Citrobacter freundii |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | 65 | activity range, profile overview | Citrobacter freundii |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | 65 | highest stability at 40°C, over 80% activity at 25-50°C, 50% activity remaining at 60°C, 30% at 65°C, profile overview | Citrobacter freundii |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
- |
Citrobacter freundii |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 9.5 | activity range, profile overview | Citrobacter freundii |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| additional information | - |
the purified enzyme is very stable at alkaline pH | Citrobacter freundii |
| 5 | 9.5 | highest stability at pH 8.5, over 80% activity at pH 6.0-9.2, 70% activity remaining at pH 9.5, 25% at pH 5.0, profile overview | Citrobacter freundii |
General Information
| General Information | Comment | Organism |
|---|---|---|
| physiological function | the purified antifungal alkaline chitinase exhibits remarkable antifungal activity against pathogenic fungi Aspergillus flavus MTCC 2798 and Aspergillus niger MTCC 9652 | Citrobacter freundii |
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