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Literature summary for 3.2.1.14 extracted from
- Purification of chitinase enzymes from Bacillus subtilis bacteria TV-125, investigation of kinetic properties and antifungal activity against Fusarium culmorum (2014), Ann. Clin. Microbiol. Antimicrob., 13, 35.
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Bacillus subtilis | - |
- |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| chitin + H2O | Bacillus subtilis | - |
? | - |
? |  |
| chitin + H2O | Bacillus subtilis TV-125 | - |
? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus subtilis | - |
isolated from leaves and root rhizospheres of plants in Turkey | - |
| Bacillus subtilis TV-125 | - |
isolated from leaves and root rhizospheres of plants in Turkey | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| native extracellular enzyme 28.4fold by ammonium sulfate fractionation and anion exchange chromatography | Bacillus subtilis |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 197.1 | - |
purified native enzyme, pH 4.0, 50°C, substrate colloidal chitin | Bacillus subtilis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| chitin + H2O | - |
Bacillus subtilis | ? | - |
? | |
| chitin + H2O | - |
Bacillus subtilis TV-125 | ? | - |
? | |
| colloidal chitin + H2O | - |
Bacillus subtilis | ? | - |
? | |
| colloidal chitin + H2O | - |
Bacillus subtilis TV-125 | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| poly-beta-1, 4-[2-acetamido-2-deoxy]-delta-glucoside glycanohydrolase | - |
Bacillus subtilis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
- |
Bacillus subtilis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4 | - |
- |
Bacillus subtilis |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 11 | activity range, 71% of maximal activity at pH 11.0 | Bacillus subtilis |
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