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Literature summary for 3.2.1.14 extracted from

  • Wang, S.L.; Liang, T.W.; Lin, B.S.; Wang, C.L.; Wu, P.C.; Liu, J.R.
    Purification and characterization of chitinase from a new species strain Pseudomonas sp. TKU008 (2010), J. Microbiol. Biotechnol., 20, 1001-1005.
    View publication on PubMed
Search for more literature for 3.2.1.14

Inhibitors

Inhibitors Comment Organism Structure
Cu2+ complete inhibition at 5 mM Pseudomonas sp.
Mn2+ complete inhibition at 5 mM Pseudomonas sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
40000
-
 x * 40000, SDS-PAGE Pseudomonas sp.

Organism

Organism UniProt Comment Textmining
Pseudomonas sp.
-
 isolated from soil in Taiwan
-
Pseudomonas sp. TKU008
-
 isolated from soil in Taiwan
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme 25fold by anion exchange chromatography, hydrophobic interaction chromatography, and gel filtration Pseudomonas sp.

Source Tissue

Source Tissue Comment Organism Textmining
cell culture culture condition suitable for production of chitinase are shaking at 30°C for 4 days in 100 ml of medium containing 1% shrimp and crab shell powder, 0.1% K2HPO4, and 0.05% MgSO4·7H2O, pH 7.0 Pseudomonas sp.
-
additional information TKU008 chitinase is suppressed by the simultaneously existing protease, which also showed the maximum activity at the fourth day of incubation Pseudomonas sp.
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.175
-
 purified enzyme, pH 7.0, 37°C Pseudomonas sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
colloidal chitin + H2O
-
 Pseudomonas sp. ?
-
 ?
colloidal chitin + H2O
-
 Pseudomonas sp. TKU008 ?
-
 ?

Subunits

Subunits Comment Organism
? x * 40000, SDS-PAGE Pseudomonas sp.
More peptide mass mapping, overview Pseudomonas sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
 Pseudomonas sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 stable up to Pseudomonas sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
 Pseudomonas sp.

pH Stability

pH Stability pH Stability Maximum Comment Organism
6 7 stable Pseudomonas sp.