Literature summary for 3.2.1.14 extracted from

Cole, D.C.; Olland, A.M.; Jacob, J.; Brooks, J.; Bursavich, M.G.; Czerwinski, R.; DeClercq, C.; Johnson, M.; Joseph-McCarthy, D.; Ellingboe, J.W.; Lin, L.; Nowak, P.; Presman, E.; Strand, J.; Tam, A.; Williams, C.M.; Yao, S.; Tsao, D.H.; Fitz, L.J.
Identification and characterization of acidic mammalian chitinase inhibitors (2010), J. Med. Chem., 53, 6122-6128.

Crystallization (Commentary)

Crystallization (Comment)	Organism
purified recombinant enzyme in complex with inhibitor 1, hanging drop vapor diffusion, 0.0002 ml of protein solution containing 0.44 mM AMCase, 0.52 mM inhibitor 1, 25 mM Tris-HCl, pH 8.0, and 50 mM NaCl, is mixed with 0.0002 ml of reservoir solution containing 20% PEG MME 5000, 100 mM bis-Tris, equilibration over 0.2 ml of reservoir solution, pH 6.5, 18°C, 2 weeks, X-ray diffraction structure determination and analysis, modeling	Homo sapiens

Crystallization (Comment)

Organism

purified recombinant enzyme in complex with inhibitor 1, hanging drop vapor diffusion, 0.0002 ml of protein solution containing 0.44 mM AMCase, 0.52 mM inhibitor 1, 25 mM Tris-HCl, pH 8.0, and 50 mM NaCl, is mixed with 0.0002 ml of reservoir solution containing 20% PEG MME 5000, 100 mM bis-Tris, equilibration over 0.2 ml of reservoir solution, pH 6.5, 18°C, 2 weeks, X-ray diffraction structure determination and analysis, modeling

Homo sapiens

Inhibitors

Inhibitors	Comment	Organism
2-(piperazin-1-yl)pyridine-3-carboxamide	-	Homo sapiens
2-methyl-3-[[4-(pyridin-2-yl)piperazin-1-yl]methyl]-1H-indole	-	Homo sapiens
4-(4-chlorophenyl)piperazine-1-carboximidamide	-	Homo sapiens
5-[4-[2-(4-bromophenoxy)ethyl]piperazin-1-yl]-1H-1,2,4-triazol-3-amine	-	Homo sapiens
N-ethyl-2-(4-methylpiperazin-1-yl)pyridine-3-carboxamide	-	Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	-	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
commercial preparation	recombinant enzyme	Homo sapiens	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
4-methylumbelliferyl-beta-D-N,N'-diacetylchitobioside + H2O	-	Homo sapiens	4-methylumbelliferone + N,N'-diacetylchitobiose	-	?

Synonyms

Synonyms	Comment	Organism
AMCase	-	Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
25	-	assay at	Homo sapiens

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
5	-	assay at	Homo sapiens