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Literature summary for 3.2.1.14 extracted from
- Differential chitinase activity and production within Francisella species, subspecies, and subpopulations (2011), J. Bacteriol., 193, 3265-3275.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression of recombinant His-tagged Chi enzymes in Escherichia coli strain BL21(DE3)/pLysS | Francisella tularensis |
| expression of recombinant His-tagged Chi enzymes in Escherichia coli strain BL21(DE3)/pLysS | Francisella tularensis subsp. novicida |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| additional information | construction of chiA and chiC knockouts strains A1 and A2 strains, respectively | Francisella tularensis |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | comparative endochitinase kinetics from different strains, overview | Francisella tularensis |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Francisella tularensis subsp. novicida | - |
- |
| intracellular | - |
Francisella tularensis | 5622 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 79000 | - |
x * 79000, recombinant His-tagged ChiB, SDS-PAGE, x * 120000, recombinant His-tagged ChiA, SDS-PAGE, x * 97000, about, recombinant His-tagged ChiA, sequence calculation | Francisella tularensis subsp. novicida |
| 97000 | - |
x * 79000, recombinant His-tagged ChiB, SDS-PAGE, x * 120000, recombinant His-tagged ChiA, SDS-PAGE, x * 97000, about, recombinant His-tagged ChiA, sequence calculation | Francisella tularensis subsp. novicida |
| 120000 | - |
x * 79000, recombinant His-tagged ChiB, SDS-PAGE, x * 120000, recombinant His-tagged ChiA, SDS-PAGE, x * 97000, about, recombinant His-tagged ChiA, sequence calculation | Francisella tularensis subsp. novicida |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Francisella tularensis | - |
several strains, genes chiA, chiB, chiC, and chiD | - |
| Francisella tularensis subsp. novicida | - |
different strins, genes chiA, chiB, chiC, and chiD | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His-tagged Chi enzymes from Escherichia coli strain BL21(DE3)/pLysS by nickel affinity chromatography | Francisella tularensis |
| recombinant His-tagged Chi enzymes from Escherichia coli strain BL21(DE3)/pLysS by nickel affinity chromatography | Francisella tularensis subsp. novicida |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
chitinase isozyme activity in different strains, overview | Francisella tularensis |
| additional information | - |
chitinase isozyme activity in different strains, overview | Francisella tularensis subsp. novicida |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-nitrophenyl N,N'-diacetyl-beta-D-chitobioside + H2O | - |
Francisella tularensis | ? | - |
? |  |
| 4-nitrophenyl N,N'-diacetyl-beta-D-chitobioside + H2O | - |
Francisella tularensis subsp. novicida | ? | - |
? | |
| 4-nitrophenyl N-acetyl-beta-D-glucosaminide + H2O | - |
Francisella tularensis | 4-nitrophenol + N-acetyl-beta-D-glucosamine | - |
? | |
| 4-nitrophenyl N-acetyl-beta-D-glucosaminide + H2O | - |
Francisella tularensis subsp. novicida | 4-nitrophenol + N-acetyl-beta-D-glucosamine | - |
? | |
| 4-nitrophenyl tri-N-acetylchitotrioside + H2O | - |
Francisella tularensis | 4-nitrophenol + tri-N-acetylchitotriose | - |
? | |
| 4-nitrophenyl tri-N-acetylchitotrioside + H2O | - |
Francisella tularensis subsp. novicida | 4-nitrophenol + tri-N-acetylchitotriose | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 79000, recombinant His-tagged ChiB, SDS-PAGE, x * 120000, recombinant His-tagged ChiA, SDS-PAGE, x * 97000, about, recombinant His-tagged ChiA, sequence calculation | Francisella tularensis subsp. novicida |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| ChiA | - |
Francisella tularensis |
| ChiA | - |
Francisella tularensis subsp. novicida |
| ChiB | - |
Francisella tularensis |
| ChiB | - |
Francisella tularensis subsp. novicida |
| ChiC | - |
Francisella tularensis |
| ChiC | - |
Francisella tularensis subsp. novicida |
| ChiD | - |
Francisella tularensis |
| ChiD | - |
Francisella tularensis subsp. novicida |
| endochitinase | - |
Francisella tularensis |
| endochitinase | - |
Francisella tularensis subsp. novicida |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 35 | - |
assay at | Francisella tularensis |
| 35 | - |
assay at | Francisella tularensis subsp. novicida |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.4 | - |
assay at | Francisella tularensis |
| 7.4 | - |
assay at | Francisella tularensis subsp. novicida |
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