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Literature summary for 3.2.1.14 extracted from

  • Fukamizo, T.; Sato, H.; Mizuhara, M.; Ohnuma, T.; Gotoh, T.; Hiwatashi, K.; Takahashi, S.
    Chitinase from Autographa californica multiple nucleopolyhedrovirus: rapid purification from Sf-9 medium and mode of action (2011), Biosci. Biotechnol. Biochem., 75, 1763-1769.
    View publication on PubMed
Search for more literature for 3.2.1.14

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
65000
-
 x * 65000, SDS-PAGE Autographa californica multiple nucleopolyhedrovirus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
N,N',N'',N''', N'''',N'''''-hexaacetylchitohexaose + 2 H2O Autographa californica multiple nucleopolyhedrovirus the enzyme hydrolyzes the second beta-1,4-glycosidic linkage from the non-reducing end of chitin oligosaccharide substrates, i.e. N,N',N'',N'''-tetraacetylchitotetraose, N,N',N'',N''',N''''-pentaacetylchitopentaose, or N,N',N'',N''', N'''',N'''''-hexaacetylchitohexaose, producing the beta-anomer of (GlcNAc)2 3 N,N'-diacetylchitobiose
-
 ?
solid beta-chitin + H2O Autographa californica multiple nucleopolyhedrovirus the enzyme hydrolyzes solid beta-chitin producing only N,N'-diacetylchitobiose N,N'-diacetylchitobiose
-
 ?

Organism

Organism UniProt Comment Textmining
Autographa californica multiple nucleopolyhedrovirus
-
 AcMNPV
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme from Sf9 cells by pepstatin-aminohexyl resin chromatography and anion exchange chromatography Autographa californica multiple nucleopolyhedrovirus

Source Tissue

Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
35.5
-
 purified enzyme, pH 7.0, 37°C Autographa californica multiple nucleopolyhedrovirus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-methylumbelliferyl N,N'-diacetylchitotriose + H2O
-
 Autographa californica multiple nucleopolyhedrovirus ?
-
 ?
additional information processing mechanism, mode of action of the enzyme, overview. The enzyme also shows transglycosylation activity Autographa californica multiple nucleopolyhedrovirus ?
-
 ?
N,N',N'',N''', N'''',N'''''-hexaacetylchitohexaose + 2 H2O the enzyme hydrolyzes the second beta-1,4-glycosidic linkage from the non-reducing end of chitin oligosaccharide substrates, i.e. N,N',N'',N'''-tetraacetylchitotetraose, N,N',N'',N''',N''''-pentaacetylchitopentaose, or N,N',N'',N''', N'''',N'''''-hexaacetylchitohexaose, producing the beta-anomer of (GlcNAc)2 Autographa californica multiple nucleopolyhedrovirus 3 N,N'-diacetylchitobiose
-
 ?
N,N',N'',N''', N'''',N'''''-hexaacetylchitohexaose + 2 H2O the enzyme hydrolyzes the second beta-1,4-glycosidic linkage from the non-reducing end of chitin oligosaccharide substrates, i.e. N,N',N'',N'''-tetraacetylchitotetraose, N,N',N'',N''',N''''-pentaacetylchitopentaose, or N,N',N'',N''', N'''',N'''''-hexaacetylchitohexaose, producing the beta-anomer of (GlcNAc)2, product analysis by MALDI-TOF mass spectrometry Autographa californica multiple nucleopolyhedrovirus 3 N,N'-diacetylchitobiose
-
 ?
solid beta-chitin + H2O the enzyme hydrolyzes solid beta-chitin producing only N,N'-diacetylchitobiose Autographa californica multiple nucleopolyhedrovirus N,N'-diacetylchitobiose
-
 ?
solid beta-chitin + H2O the enzyme hydrolyzes solid beta-chitin producing only N,N'-diacetylchitobiose, product analysis by MALDI-TOF mass spectrometry Autographa californica multiple nucleopolyhedrovirus N,N'-diacetylchitobiose
-
 ?

Subunits

Subunits Comment Organism
? x * 65000, SDS-PAGE Autographa californica multiple nucleopolyhedrovirus

Synonyms

Synonyms Comment Organism
endo-chitinase
-
 Autographa californica multiple nucleopolyhedrovirus
More the enzyme is a GH18 family chitinase Autographa californica multiple nucleopolyhedrovirus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Autographa californica multiple nucleopolyhedrovirus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 assay at Autographa californica multiple nucleopolyhedrovirus

General Information

General Information Comment Organism
physiological function the viral chitinase is involved in the final liquefaction of the infected host larvae Autographa californica multiple nucleopolyhedrovirus