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Literature summary for 3.2.1.14 extracted from
- Identification of chitinases Is-chiA and Is-chiB from Isoptericola jiangsuensis CLG and their characterization (2011), Appl. Microbiol. Biotechnol., 89, 705-713.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene chiB, DNA and amino acid sequence determination and analysis, expression of His-tagged Is-chiB in Escherichia coli strain BL21 (DE3), subcloning in Escherichia coli strain DH5alpha | Isoptericola jiangsuensis |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | inhibits slightly at 10 mM | Isoptericola jiangsuensis |  |
| Cr3+ | inhibits slightly at 10 mM | Isoptericola jiangsuensis | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| additional information | the enzyme is not affected by Ca2+, Ba2+, Zn2+, Li+, Mn2+, Mg2+, Cu2+, Fe3+, Pb2+, and Cd2+ | Isoptericola jiangsuensis |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 59330 | - |
x * 60000, recombinant enzyme, SDS-PAGE, x * 59330, sequence calculation | Isoptericola jiangsuensis |
| 60000 | - |
x * 60000, recombinant enzyme, SDS-PAGE, x * 59330, sequence calculation | Isoptericola jiangsuensis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Isoptericola jiangsuensis | D3YGV3 | DSM 21863, CCTCC AB208287, gene chiB encoding isozyme Is-chiB | - |
| Isoptericola jiangsuensis CLG | D3YGV3 | DSM 21863, CCTCC AB208287, gene chiB encoding isozyme Is-chiB | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His-tagged Is-chiB from Escherichia coli strain BL21 (DE3) by nickel affinity chromatography and ultrafiltration | Isoptericola jiangsuensis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-methylumbelliferyl N,N',N''-triacetylchitotriose + H2O | - |
Isoptericola jiangsuensis | ? | - |
? | |
| 4-methylumbelliferyl N,N',N''-triacetylchitotriose + H2O | - |
Isoptericola jiangsuensis CLG | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 60000, recombinant enzyme, SDS-PAGE, x * 59330, sequence calculation | Isoptericola jiangsuensis |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| endochitinase | - |
Isoptericola jiangsuensis |
| Is-chiB | - |
Isoptericola jiangsuensis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
- |
Isoptericola jiangsuensis |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 20 | 90 | activity range, profile, overview | Isoptericola jiangsuensis |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
purified recombinant enzyme, stable below | Isoptericola jiangsuensis |
| 50 | - |
purified recombinant enzyme, 1 h, 30% activity remaining | Isoptericola jiangsuensis |
| 80 | - |
purified recombinant enzyme, 1 h, complete inactivation | Isoptericola jiangsuensis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 9 | - |
- |
Isoptericola jiangsuensis |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 3 | 10 | activity range, profile, overview | Isoptericola jiangsuensis |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 3 | - |
purified recombinant enzyme, 1 h, about 5% activity remaining | Isoptericola jiangsuensis |
| 5 | - |
purified recombinant enzyme, 1 h, 50% activity remaining | Isoptericola jiangsuensis |
| 7 | 10 | purified recombinant enzyme, 1 h, over 80% activity remaining, most stable at pH 8.5 | Isoptericola jiangsuensis |
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