General Stability | Organism |
---|---|
the enzyme exhibits higher stability in presence of commonly used protectant fungicides Captan, Carbendazim, and Mancozeb compared to control | Paenibacillus sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | determination of kinetic constants and thermodynamic parameters of the enzyme | Paenibacillus sp. |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Paenibacillus sp. | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
56560 | - |
x * 56560, SDS-PAGE | Paenibacillus sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Paenibacillus sp. | - |
- |
- |
Paenibacillus sp. D1 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
native enzyme, 52.3fold by ammonium sulfate fractionation and ion exchange chromatography | Paenibacillus sp. |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
492.4 | - |
purified enzyme, pH 5.0, 50°C | Paenibacillus sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
chitin + H2O | the enzyme shows a high affinity of the enzyme for chitin | Paenibacillus sp. | ? | - |
? | |
chitin + H2O | the enzyme shows a high affinity of the enzyme for chitin | Paenibacillus sp. D1 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 56560, SDS-PAGE | Paenibacillus sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
- |
Paenibacillus sp. |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | 60 | activity range | Paenibacillus sp. |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
purified enzyme, half-life is 370.68 min | Paenibacillus sp. |
50 | - |
purified enzyme, half-life is 255.15 min | Paenibacillus sp. |
80 | - |
purified enzyme, half-life is 22.61 min | Paenibacillus sp. |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
83.46 | - |
chitin | pH 5.0, 50°C | Paenibacillus sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | - |
- |
Paenibacillus sp. |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
3 | 10 | 85.63% of maximal activity at pH 4.0, maximal activity at pH 5.0, and 90.61% of maximal activity pH 8.0 | Paenibacillus sp. |
General Information | Comment | Organism |
---|---|---|
physiological function | chitinases are glycoside hydrolases that catalyze the degradation of chitin, a linear beta-1,4-linked polymer of N-acetylglucosamine | Paenibacillus sp. |