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Literature summary for 3.2.1.14 extracted from

  • Singh, A.K.; Chhatpar, H.S.
    Purification and characterization of chitinase from Paenibacillus sp. D1 (2011), Appl. Biochem. Biotechnol., 164, 77-88.
    View publication on PubMed
Search for more literature for 3.2.1.14

General Stability

General Stability Organism
the enzyme exhibits higher stability in presence of commonly used protectant fungicides Captan, Carbendazim, and Mancozeb compared to control Paenibacillus sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information determination of kinetic constants and thermodynamic parameters of the enzyme Paenibacillus sp.

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Paenibacillus sp.
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
56560
-
 x * 56560, SDS-PAGE Paenibacillus sp.

Organism

Organism UniProt Comment Textmining
Paenibacillus sp.
-
-
-
Paenibacillus sp. D1
-
-
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme, 52.3fold by ammonium sulfate fractionation and ion exchange chromatography Paenibacillus sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
492.4
-
 purified enzyme, pH 5.0, 50°C Paenibacillus sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
chitin + H2O the enzyme shows a high affinity of the enzyme for chitin Paenibacillus sp. ?
-
 ?
chitin + H2O the enzyme shows a high affinity of the enzyme for chitin Paenibacillus sp. D1 ?
-
 ?

Subunits

Subunits Comment Organism
? x * 56560, SDS-PAGE Paenibacillus sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
 Paenibacillus sp.

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
25 60 activity range Paenibacillus sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
 purified enzyme, half-life is 370.68 min Paenibacillus sp.
50
-
 purified enzyme, half-life is 255.15 min Paenibacillus sp.
80
-
 purified enzyme, half-life is 22.61 min Paenibacillus sp.

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
83.46
-
 chitin pH 5.0, 50°C Paenibacillus sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
-
 Paenibacillus sp.

pH Range

pH Minimum pH Maximum Comment Organism
3 10 85.63% of maximal activity at pH 4.0, maximal activity at pH 5.0, and 90.61% of maximal activity pH 8.0 Paenibacillus sp.

General Information

General Information Comment Organism
physiological function chitinases are glycoside hydrolases that catalyze the degradation of chitin, a linear beta-1,4-linked polymer of N-acetylglucosamine Paenibacillus sp.