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Literature summary for 3.2.1.14 extracted from

  • Mi, Q.; Yang, J.; Ye, F.; Gan, Z.; Wu, C.; Niu, X.; Zou, C.; Zhang, K.
    Cloning and overexpression of Pochonia chlamydosporia chitinase gene pcchi44, a potential virulence factor in infection against nematodes (2010), Process Biochem., 45, 810-814.
No PubMed abstract available
Search for more literature for 3.2.1.14

Cloned(Commentary)

Cloned (Comment) Organism
-
 Pochonia chlamydosporia

Inhibitors

Inhibitors Comment Organism Structure
acetazolamide complete inhibition Pochonia chlamydosporia

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00965
-
 4-methylumbelliferyl-beta-D-N,N',N''-triacetylchitotrioside pH 5.5, 42°C Pochonia chlamydosporia

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Pochonia chlamydosporia
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
44000
-
 x * 44000, SDS-PAGE Pochonia chlamydosporia

Organism

Organism UniProt Comment Textmining
Pochonia chlamydosporia B5L0W0
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein sequence contains two putative N-glycosylation sites Pochonia chlamydosporia
proteolytic modification the putative primary signal peptidase cleavage site is between residues A22 and T23, and the second processing step is downstream to R35 and A36 Pochonia chlamydosporia

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
25.85
-
 pH 5.5, 42°C Pochonia chlamydosporia

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-methylumbelliferyl beta-D-N,N',N''-triacetylchitotrioside + H2O
-
 Pochonia chlamydosporia 4-methylumbelliferol + N,N',N''-triacetylchitotriose
-
 ?

Subunits

Subunits Comment Organism
? x * 44000, SDS-PAGE Pochonia chlamydosporia

Synonyms

Synonyms Comment Organism
Chi44
-
 Pochonia chlamydosporia

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
 Pochonia chlamydosporia

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
 Pochonia chlamydosporia

General Information

General Information Comment Organism
physiological function chitinase can damage eggs of both the root-knot nematode Meloidogyne incognita and the insect Bombyx mori Pochonia chlamydosporia