Protein Variants | Comment | Organism |
---|---|---|
W275G | mutation significantly shifts the anomer seelectivity of the enzyme toward beta substrates. 5- to 10fold decrease in kcat value and kcat/Km ratio for all substrates tested | Vibrio harveyi |
W397F | mutation significantly shifts the anomer seelectivity of the enzyme toward beta substrates and weakens the binding strength at subsite +2 | Vibrio harveyi |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.174 | - |
GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAc | wild-type, 37°C | Vibrio harveyi | |
0.238 | - |
GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAc | mutant W275G, 37°C | Vibrio harveyi | |
0.315 | - |
GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAc | mutant W275G, 37°C | Vibrio harveyi | |
0.38 | - |
GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAc | wild-type, 37°C | Vibrio harveyi | |
0.46 | - |
GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAc | mutant W397F, 37°C | Vibrio harveyi | |
0.476 | - |
GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAc | mutant W397F, 37°C | Vibrio harveyi |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Vibrio harveyi | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
colloidal chitin + H2O | - |
Vibrio harveyi | ? | - |
? | |
crystalline alpha-chitin + H2O | - |
Vibrio harveyi | GlcNAcbeta(1-4)GlcNAcbeta + GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAc + GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)-GlcNAc + GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta + GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAc | - |
? | |
GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAc + H2O | i.e. (GlcNAc)6 | Vibrio harveyi | GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAc + GlcNAcbeta(1-4)GlcNAc | - |
? | |
GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAc + H2O | i.e. (GlcNAc)6 | Vibrio harveyi | GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAc + GlcNAcbeta(1-4)GlcNAcbeta + GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAc | - |
? | |
additional information | substrate (GlcNAc)6 prefers subsites -2 to +2 over subsites -3 to +2. Substrate (GlcNAc)5 only requires subsites -2 to +2. The binding of the chitooligosaccharides to the enzyme essentially occurs in compulsory fashion | Vibrio harveyi | ? | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.02 | - |
colloidal chitin | mutant W275G, 37°C | Vibrio harveyi | |
0.03 | - |
colloidal chitin | mutant W397F, 37°C | Vibrio harveyi | |
0.04 | - |
GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAc | mutant W275G, 37°C | Vibrio harveyi | |
0.06 | - |
GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAc | mutant W275G, 37°C | Vibrio harveyi | |
0.1 | - |
colloidal chitin | wild-type, 37°C | Vibrio harveyi | |
0.19 | - |
GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAc | wild-type, 37°C | Vibrio harveyi | |
0.21 | - |
GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAc | wild-type, 37°C | Vibrio harveyi | |
2.1 | - |
GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAc | mutant W397F, 37°C | Vibrio harveyi | |
3 | - |
GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAc | mutant W397F, 37°C | Vibrio harveyi |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.13 | - |
GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAc | mutant W275G, 37°C | Vibrio harveyi | |
0.25 | - |
GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAc | mutant W275G, 37°C | Vibrio harveyi | |
0.55 | - |
GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAc | wild-type, 37°C | Vibrio harveyi | |
1.1 | - |
GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAc | wild-type, 37°C | Vibrio harveyi | |
4.4 | - |
GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAc | mutant W397F, 37°C | Vibrio harveyi | |
6.5 | - |
GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAc | mutant W397F, 37°C | Vibrio harveyi |