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Literature summary for 3.2.1.14 extracted from

  • Suginta, W.; Pantoom, S.; Prinz, H.
    Substrate binding modes and anomer selectivity of chitinase A from Vibrio harveyi (2009), J. Chem. Biol., 2, 191-202.
    View publication on PubMedView publication on EuropePMC

Protein Variants

Protein Variants Comment Organism
W275G mutation significantly shifts the anomer seelectivity of the enzyme toward beta substrates. 5- to 10fold decrease in kcat value and kcat/Km ratio for all substrates tested Vibrio harveyi
W397F mutation significantly shifts the anomer seelectivity of the enzyme toward beta substrates and weakens the binding strength at subsite +2 Vibrio harveyi

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.174
-
GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAc wild-type, 37°C Vibrio harveyi
0.238
-
GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAc mutant W275G, 37°C Vibrio harveyi
0.315
-
GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAc mutant W275G, 37°C Vibrio harveyi
0.38
-
GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAc wild-type, 37°C Vibrio harveyi
0.46
-
GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAc mutant W397F, 37°C Vibrio harveyi
0.476
-
GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAc mutant W397F, 37°C Vibrio harveyi

Organism

Organism UniProt Comment Textmining
Vibrio harveyi
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
colloidal chitin + H2O
-
Vibrio harveyi ?
-
?
crystalline alpha-chitin + H2O
-
Vibrio harveyi GlcNAcbeta(1-4)GlcNAcbeta + GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAc + GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)-GlcNAc + GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta + GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAc
-
?
GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAc + H2O i.e. (GlcNAc)6 Vibrio harveyi GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAc + GlcNAcbeta(1-4)GlcNAc
-
?
GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAc + H2O i.e. (GlcNAc)6 Vibrio harveyi GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAc + GlcNAcbeta(1-4)GlcNAcbeta + GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAc
-
?
additional information substrate (GlcNAc)6 prefers subsites -2 to +2 over subsites -3 to +2. Substrate (GlcNAc)5 only requires subsites -2 to +2. The binding of the chitooligosaccharides to the enzyme essentially occurs in compulsory fashion Vibrio harveyi ?
-
?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.02
-
colloidal chitin mutant W275G, 37°C Vibrio harveyi
0.03
-
colloidal chitin mutant W397F, 37°C Vibrio harveyi
0.04
-
GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAc mutant W275G, 37°C Vibrio harveyi
0.06
-
GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAc mutant W275G, 37°C Vibrio harveyi
0.1
-
colloidal chitin wild-type, 37°C Vibrio harveyi
0.19
-
GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAc wild-type, 37°C Vibrio harveyi
0.21
-
GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAc wild-type, 37°C Vibrio harveyi
2.1
-
GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAc mutant W397F, 37°C Vibrio harveyi
3
-
GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAc mutant W397F, 37°C Vibrio harveyi

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.13
-
GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAc mutant W275G, 37°C Vibrio harveyi
0.25
-
GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAc mutant W275G, 37°C Vibrio harveyi
0.55
-
GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAc wild-type, 37°C Vibrio harveyi
1.1
-
GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAc wild-type, 37°C Vibrio harveyi
4.4
-
GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAc mutant W397F, 37°C Vibrio harveyi
6.5
-
GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAc mutant W397F, 37°C Vibrio harveyi