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Literature summary for 3.2.1.14 extracted from
- Cloning, expression and purification of Bacillus cereus endochitinase in the Escherichia coli AD494(DE3)pLysS expression system (2009), Biosci. Biotechnol. Biochem., 73, 1172-1174.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| 2-mercaptoethanol | - |
Bacillus cereus |  |
| Urea | - |
Bacillus cereus | |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Bacillus cereus |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | activation | Bacillus cereus | |
| Mn2+ | activation | Bacillus cereus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 69600 | - |
x * 74500, calculated, x * 69600, SDS-PAGE of recombinant enzyme | Bacillus cereus |
| 74500 | - |
x * 74500, calculated, x * 69600, SDS-PAGE of recombinant enzyme | Bacillus cereus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus cereus | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| proteolytic modification | amino acid sequence lacks 33 N-terminal amino acids | Bacillus cereus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-nitrophenyl beta-D-GlcNAc + H2O | 0.8% of the activity with 4-nitrophenyl-(beta-D-GlcNAc)3 | Bacillus cereus | 4-nitrophenol + N-acetyl-D-glucosamine | - |
? | |
| 4-nitrophenyl-GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAc + H2O | - |
Bacillus cereus | 4-nitrophenol + GlcNAcbeta(1-4)GlcNAcbeta(1-4)GlcNAc | - |
? | |
| carboxymethyl cellulose + H2O | 1.9% of the activity with colloidal chitin | Bacillus cereus | ? | - |
? | |
| chitin powder + H2O | 16.6% of the activity with colloidal chitin | Bacillus cereus | ? | - |
? | |
| chitosan + H2O | 45.2% of the activity with colloidal chitin | Bacillus cereus | ? | - |
? | |
| colloidal chitin + H2O | - |
Bacillus cereus | ? | - |
? | |
| glycol chitosan + H2O | 3.0% of the activity with colloidal chitin | Bacillus cereus | ? | - |
? | |
| additional information | no substrate: cellulose, Avicel PH-101 | Bacillus cereus | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 74500, calculated, x * 69600, SDS-PAGE of recombinant enzyme | Bacillus cereus |
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