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Literature summary for 3.2.1.14 extracted from
- Two novel thermostable chitinase genes from thermophilic fungi: cloning, expression and characterization (2010), Biores. Technol., 101, 5546-5551.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Pichia pastoris | Thermoascus aurantiacus |
| expression in Pichia pastoris | Thermochaetoides thermophila |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 47300 | - |
x * 47300, SDS-PAGE | Thermochaetoides thermophila |
| 48400 | - |
x * 48400, SDS-PAGE | Thermoascus aurantiacus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Thermoascus aurantiacus | A6YJX1 | var. levisporus | - |
| Thermochaetoides thermophila | B3GGC7 | - |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| chitosan + H2O | 11.8% of the activity with colloidal chitin | Thermoascus aurantiacus | chitosan oligosaccharides | - |
? |  |
| chitosan + H2O | 17.4% of the activity with colloidal chitin | Thermochaetoides thermophila | chitosan oligosaccharides | - |
? | |
| colloidal chitin + H2O | - |
Thermoascus aurantiacus | GlcNAcbeta(1-4)GlcNAc + ? | major product | ? | |
| colloidal chitin + H2O | - |
Thermochaetoides thermophila | GlcNAcbeta(1-4)GlcNAc + ? | major product | ? | |
| additional information | no substrate: 4-nitrophenyl-(GlcNAc) | Thermoascus aurantiacus | ? | - |
? | |
| additional information | no substrate: 4-nitrophenyl-(GlcNAc) | Thermochaetoides thermophila | ? | - |
? | |
| powdery chitin + H2O | 15.4% of the activity with colloidal chitin | Thermoascus aurantiacus | ? | - |
? | |
| powdery chitin + H2O | 32.2% of the activity with colloidal chitin | Thermochaetoides thermophila | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 47300, SDS-PAGE | Thermochaetoides thermophila |
| ? | x * 48400, SDS-PAGE | Thermoascus aurantiacus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
- |
Thermoascus aurantiacus |
| 60 | - |
- |
Thermochaetoides thermophila |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
60 min, 95% residual activity | Thermoascus aurantiacus |
| 60 | - |
60 min, 96.7% residual activity | Thermochaetoides thermophila |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | - |
- |
Thermochaetoides thermophila |
| 8 | - |
- |
Thermoascus aurantiacus |
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Release 2023.1 (January 2023)
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