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Literature summary for 3.2.1.14 extracted from

  • Islam, S.A.; Cho, K.M.; Hong, S.J.; Math, R.K.; Kim, J.M.; Yun, M.G.; Cho, J.J.; Heo, J.Y.; Lee, Y.H.; Kim, H.; Yun, H.D.
    Chitinase of Bacillus licheniformis from oyster shell as a probe to detect chitin in marine shells (2010), Appl. Microbiol. Biotechnol., 86, 119-129.
    View publication on PubMed
Search for more literature for 3.2.1.14

Cloned(Commentary)

Cloned (Comment) Organism
-
 Bacillus licheniformis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.009
-
 4-methylumbelliferyl beta-N,N'-diacetylchitobioside pH 8.0, 55°C Bacillus licheniformis
0.129
-
 alpha-chitin pH 8.0, 55°C Bacillus licheniformis

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+ 25 mM, about 80% stimulation Bacillus licheniformis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
62000
-
 x * 62000, SDS-PAGE, x * 62135, calculated Bacillus licheniformis
62135
-
 x * 62000, SDS-PAGE, x * 62135, calculated Bacillus licheniformis

Organism

Organism UniProt Comment Textmining
Bacillus licheniformis Q1EM71 isoform ChiB
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-methylumbelliferyl beta-N,N'-diacetylchitobioside + H2O
-
 Bacillus licheniformis 4-methylumbelliferol + beta-N,N'-diacetylchitobiose
-
 ?
alpha-chitin + H2O
-
 Bacillus licheniformis ?
-
 ?

Subunits

Subunits Comment Organism
? x * 62000, SDS-PAGE, x * 62135, calculated Bacillus licheniformis
More enzyme consists of a catalytic domain, a fibronectin type III domain , and a chitin binding domain. The catalytic domain with one amino acid in C-terminal region is sufficient for chitinase activity. The C-terminus is important in binduing to shell powder Bacillus licheniformis

Synonyms

Synonyms Comment Organism
Chi18B
-
 Bacillus licheniformis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
55
-
-
 Bacillus licheniformis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
-
 Bacillus licheniformis

General Information

General Information Comment Organism
physiological function enzyme consists of a catalytic domain, a fibronectin type III domain, and a chitin binding domain. The catalytic domain with one amino acid in C-terminal region is sufficient for chitinase activity. The C-terminus is important in binduing to shell powder Bacillus licheniformis