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Literature summary for 3.2.1.14 extracted from
- Oat (Avena sativa) seed extract as an antifungal food preservative through the catalytic activity of a highly abundant class I chitinase (2009), Appl. Biochem. Biotechnol., 160, 1573-1584.
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 34000 | - |
x * 34000, SDS-PAGE | Hordeum vulgare |
| 34000 | - |
x * 34000, SDS-PAGE | Secale cereale |
| 34000 | - |
x * 34000, SDS-PAGE | Triticum aestivum |
| 34000 | - |
x * 34000, SDS-PAGE | Avena sativa |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Avena sativa | the class I chitinase inhibits the growth of Penicillium roqueforti IBT 5426, overview | ? | - |
? |  |
| additional information | Hordeum vulgare | the class I chitinase inhibits the growth of Penicillium roquefortii, overview | ? | - |
? | |
| additional information | Secale cereale | the class I chitinase inhibits the growth of Penicillium roquefortii, overview | ? | - |
? | |
| additional information | Triticum aestivum | the class I chitinase inhibits the growth of Penicillium roquefortii, overview | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Avena sativa | P86181 | cv. Adamo and Markant | - |
| Hordeum vulgare | P11955 | cv. Smilla | - |
| Hordeum vulgare | Q42839 | cv. Smilla | - |
| Secale cereale | Q9AXR9 | - |
- |
| Secale cereale | Q9FRV1 | - |
- |
| Triticum aestivum | Q41539 | Chi | - |
| Triticum aestivum | Q6T484 | - |
- |
| Triticum aestivum | Q8W427 | Chi3 | - |
| Triticum aestivum | Q8W428 | Chi2 | - |
| Triticum aestivum | Q9XEN6 | Cht4 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| native enzyme from seed extract by anion exchange and hydrophobic interaction exchange chromatography | Avena sativa |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| seed | - |
Hordeum vulgare | - |
| seed | - |
Secale cereale | - |
| seed | - |
Triticum aestivum | - |
| seed | - |
Avena sativa | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
enzyme in oat seed extract | Avena sativa |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | the class I chitinase inhibits the growth of Penicillium roqueforti IBT 5426, overview | Avena sativa | ? | - |
? | |
| additional information | the class I chitinase inhibits the growth of Penicillium roquefortii, overview | Hordeum vulgare | ? | - |
? | |
| additional information | the class I chitinase inhibits the growth of Penicillium roquefortii, overview | Secale cereale | ? | - |
? | |
| additional information | the class I chitinase inhibits the growth of Penicillium roquefortii, overview | Triticum aestivum | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 34000, SDS-PAGE | Hordeum vulgare |
| ? | x * 34000, SDS-PAGE | Secale cereale |
| ? | x * 34000, SDS-PAGE | Triticum aestivum |
| ? | x * 34000, SDS-PAGE | Avena sativa |
| More | determination of the primary structure by mass spectrometry, sequence comparisons, overview | Hordeum vulgare |
| More | determination of the primary structure by mass spectrometry, sequence comparisons, overview | Secale cereale |
| More | determination of the primary structure by mass spectrometry, sequence comparisons, overview | Triticum aestivum |
| More | determination of the primary structure by mass spectrometry, sequence comparisons, overview | Avena sativa |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| CHI | - |
Triticum aestivum |
| CHI2 | - |
Triticum aestivum |
| Chi3 | - |
Triticum aestivum |
| Cht4 | - |
Triticum aestivum |
| class I chitinase | - |
Hordeum vulgare |
| class I chitinase | - |
Secale cereale |
| class I chitinase | - |
Triticum aestivum |
| class I chitinase | - |
Avena sativa |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
- |
Hordeum vulgare |
| 7 | - |
- |
Secale cereale |
| 7 | - |
- |
Triticum aestivum |
| 7 | - |
- |
Avena sativa |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Hordeum vulgare | two basic isoforms with pI values of 7.6 and 8.0 | - |
7.6 |
| Secale cereale | two basic isoforms with pI values of 7.6 and 8.0 | - |
7.6 |
| Triticum aestivum | two basic isoforms with pI values of 7.6 and 8.0 | - |
7.6 |
| Avena sativa | two basic isoforms with pI values of 7.6 and 8.0 | - |
7.6 |
| Hordeum vulgare | two basic isoforms with pI values of 7.6 and 8.0 | - |
8 |
| Secale cereale | two basic isoforms with pI values of 7.6 and 8.0 | - |
8 |
| Triticum aestivum | two basic isoforms with pI values of 7.6 and 8.0 | - |
8 |
| Avena sativa | two basic isoforms with pI values of 7.6 and 8.0 | - |
8 |
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